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EQUIPE 1 :SIGNAUX PROTÉOLYTIQUES DE L'INTESTIN
TEAM 1 : GUT PROTEASE SIGNALS |
Mini CV
- 2016-présent : Chercheur statutaire, INSERM Unité 1220, Institut de Recherche en Santé Digestive IRSD
- 2011-2015: Chercheur statutaire, UMR INSERM U1043/ CNRS U5282
- 2002-2010: Chercheur statutaire, INSERM Unité 563
- 1993-2002: Chercheur statutaire, INSERM Unité 326
- 2016-present : Full position researcher at INSERM Unit 1220, Institut de Recherche en Santé Digestive IRSD
- 2011-2015: Full position researcher at UMR INSERM U1043/ CNRS U5282
- 2002-2010: Full position researcher at INSERM Unit 563
- 1993-2002: Full position researcher at INSERM Unit 326
expertise
- Cellules intestinales primaires humaines et murines cultivées sous forme d’organoïde (Tri de cellules souches, co-culture avec le stroma)
- Lignées cellulaires cancéreuses cultivées sous forme de sphéroïdes
- Tissus de souris PAR2 KO
- Etudes en transduction du signal par les techniques de biochimie des protéines et des lipides
- Human and mouse primary intestinal cells cultured as organoids (Stem cell sorting, co-culture with stroma)
- Cancer cell lines cultured as spheroids
- PAR2 KO tissues
- Signal transduction studies (protein and lipid biochemistry)
Recherche/projet research/project
Cellules souches, adhésion cellulaire et transduction du signal
Les cellules souches intestinales (CSI) ont récemment été identifiées et impliquées dans les pathologies inflammatoires et cancéreuses intestinales. Notre objectif est de mieux appréhender les différents sous-types de CSI et leurs fonctions en physiologie et physiopathologie. En particulier, nous étudions la signalisation croisée entre les récepteurs d’adhésion et les autres récepteurs membranaires tels que les récepteurs de protéases inflammatoires
Stem cells, cell adhesion and signal transduction
Intestinal stem cells (ISC) have recently been identified and implicated in both inflammatory and cancer intestinal pathologies. Our aim is to get a better understanding of ISC sub-types and functions in both physiological and pathological conditions. Specially, we investigate the signaling crosstalk between adhesion receptors and other receptors such as protease-activated receptors.
Les cellules souches intestinales (CSI) ont récemment été identifiées et impliquées dans les pathologies inflammatoires et cancéreuses intestinales. Notre objectif est de mieux appréhender les différents sous-types de CSI et leurs fonctions en physiologie et physiopathologie. En particulier, nous étudions la signalisation croisée entre les récepteurs d’adhésion et les autres récepteurs membranaires tels que les récepteurs de protéases inflammatoires
Stem cells, cell adhesion and signal transduction
Intestinal stem cells (ISC) have recently been identified and implicated in both inflammatory and cancer intestinal pathologies. Our aim is to get a better understanding of ISC sub-types and functions in both physiological and pathological conditions. Specially, we investigate the signaling crosstalk between adhesion receptors and other receptors such as protease-activated receptors.
Production scientifique scientific production
Intestinal organoids to model Salmonella infection and its impact on progenitors.
Yan J, Racaud-Sultan C, Pezier T, Edir A, Rolland C, Claverie C, Burlaud-Gaillard J, Olivier M, Velge P, Lacroix-Lamandé S, Vergnolle N, Wiedemann A. Sci Rep. 2024 Jul 2;14(1):15160.
Daphnanes diterpenes from the latex of Hura crepitans L. and their PKCζ-dependent anti-proliferative activity on colorectal cancer cells.
Crossay E, Jullian V, Trinel M, Sagnat D, Hamel D, Groppi E, Rolland C, Stigliani JL, Mejia K, Cabanillas BJ, Alric L, Buscail E, El Kalamouni C, Mavingui P, Deraison C, Racaud-Sultan C, Fabre N. Bioorg Med Chem. 2023 Jul 15;90:117366.
Prenatal stress induces changes in PAR2- and M3-dependent regulation of colon primitive cells.
Berger M, Guiraud L, Dumas A, Sagnat D, Payros G, Rolland C, Vergnolle N, Deraison C, Cenac N, Racaud-Sultan C. Am J Physiol Gastrointest Liver Physiol. 2022 Dec 1;323(6):G609-G626
GSK3β, a Master Kinase in the Regulation of Adult Stem Cell Behavior.
Racaud-Sultan C, Vergnolle N. Cells. 2021 Jan 24;10(2):225. doi: 10.3390/cells10020225. PMID: 33498808 Free PMC article. Review.
Daphnanes diterpenes from the latex of Hura crepitans L. And activity against human colorectal cancer cells Caco-2.
Trinel M, Le Lamer AC, Jullian V, Jacquemin D, Graton J, Cristofoli V, Crossay E, Yassine M, Rolland C, Vergnolle N, Mejia K, Joel Cabanillas B, Racaud-Sultan C, Fabre N. Bioorg Chem. 2020 Oct;103:104132. doi: 10.1016/j.bioorg.2020.104132. Epub 2020 Jul 23. PMID: 32768743
Characterization of Human Colon Organoids From Inflammatory Bowel Disease Patients.
d'Aldebert E, Quaranta M, Sébert M, Bonnet D, Kirzin S, Portier G, Duffas JP, Chabot S, Lluel P, Allart S, Ferrand A, Alric L, Racaud-Sultan C, Mas E, Deraison C, Vergnolle N. Front Cell Dev Biol. 2020 Jun 4;8:363. doi: 10.3389/fcell.2020.00363. eCollection 2020. PMID: 32582690 Free PMC article.
Sexual dimorphism in PAR2-dependent regulation of primitive colonic cells.
Noguerol J, Roustan PJ, N'Taye M, Delcombel L, Rolland C, Guiraud L, Sagnat D, Edir A, Bonnart C, Denadai-Souza A, Deraison C, Vergnolle N, Racaud-Sultan C. Biol Sex Differ. 2019 Sep 6;10(1):47. doi: 10.1186/s13293-019-0262-6. PMID: 31492202 Free PMC article.
FAK alternative splice mRNA variants expression pattern in colorectal cancer.
Devaud C, Tilkin-Mariamé AF, Vignolle-Vidoni A, Souleres P, Denadai-Souza A, Rolland C, Duthoit C, Blanpied C, Chabot S, Bouillé P, Lluel P, Vergnolle N, Racaud-Sultan C, Ferrand A. Int J Cancer. 2019 Jul 15;145(2):494-502. doi: 10.1002/ijc.32120. Epub 2019 Feb 14. PMID: 30628725 Free PMC article.
Sustainable Positive Response to Sirolimus in Juvenile Polyposis of Infancy.
Quaranta M, Laborde N, Ferrand A, Danjoux M, Vergnolle N, Barreau F, Racaud-Sultan C, Mas E. J Pediatr Gastroenterol Nutr. 2019 Feb;68(2):e38-e40. doi: 10.1097/MPG.0000000000002179. PMID: 30334931 No abstract available.
Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a protease-activated receptor 1- and protease-activated receptor 4-dependent mechanism.
Sébert M, Denadai-Souza A, Quaranta M, Racaud-Sultan C, Chabot S, Lluel P, Monjotin N, Alric L, Portier G, Kirzin S, Bonnet D, Ferrand A, Vergnolle N. Br J Pharmacol. 2018 Sep;175(18):3656-3668. doi: 10.1111/bph.14430. Epub 2018 Aug 7. PMID: 29959891 Free PMC article.
[Progress in prostate cancer study: 3D cell culture enables the ex vivo reproduction of tumor characteristics].
Tostivint V, Racaud-Sultan C, Roumiguié M, Soulié M, Gamé X, Beauval JB. Presse Med. 2017 Oct;46(10):954-965. doi: 10.1016/j.lpm.2017.06.014. Epub 2017 Sep 28. PMID: 28967525 French.
Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells.
Nasri I, Chawech R, Girardi C, Mas E, Ferrand A, Vergnolle N, Fabre N, Mezghani-Jarraya R, Racaud-Sultan C. Pharm Biol. 2017 Dec;55(1):124-131. doi: 10.1080/13880209.2016.1230877. Epub 2016 Sep 22. PMID: 27925497 Free PMC article.
PAR2-dependent activation of GSK3β regulates the survival of colon stem/progenitor cells.
Nasri I, Bonnet D, Zwarycz B, d'Aldebert E, Khou S, Mezghani-Jarraya R, Quaranta M, Rolland C, Bonnart C, Mas E, Ferrand A, Cenac N, Magness S, Van Landeghem L, Vergnolle N, Racaud-Sultan C. Am J Physiol Gastrointest Liver Physiol. 2016 Aug 1;311(2):G221-36. doi: 10.1152/ajpgi.00328.2015. Epub 2016 Jun 16. PMID: 27313176 Free PMC article.
Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered Wnt signaling.
Despeaux M, Chicanne G, Rouer E, De Toni-Costes F, Bertrand J, Mansat-De Mas V, Vergnolle N, Eaves C, Payrastre B, Girault JA, Racaud-Sultan C. Stem Cells. 2012 Aug;30(8):1597-610. doi: 10.1002/stem.1157. PMID: 22714993
Sex differences in the GSK3β-mediated survival of adherent leukemic progenitors.
Bertrand J, Despeaux M, Joly S, Bourogaa E, Gallay N, Demur C, Bonnevialle P, Louache F, Maguer-Satta V, Vergnolle N, Payrastre B, Racaud-Sultan C. Oncogene. 2012 Feb 9;31(6):694-705. doi: 10.1038/onc.2011.258. Epub 2011 Jul 4. PMID: 21725365
Critical features of FAK-expressing AML bone marrow microenvironment through leukemia stem cell hijacking of mesenchymal stromal cells.
Despeaux M, Labat E, Gadelorge M, Prade N, Bertrand J, Demur C, Recher C, Bonnevialle P, Payrastre B, Bourin P, Racaud-Sultan C. Leukemia. 2011 Nov;25(11):1789-93. doi: 10.1038/leu.2011.145. Epub 2011 Jun 7. PMID: 21647157 No abstract available.
A New alpha5beta1 integrin-dependent survival pathway through GSK3beta activation in leukemic cells.
De Toni-Costes F, Despeaux M, Bertrand J, Bourogaa E, Ysebaert L, Payrastre B, Racaud-Sultan C. PLoS One. 2010 Mar 23;5(3):e9807. doi: 10.1371/journal.pone.0009807. PMID: 20352103 Free PMC article.
A crosstalk between the Wnt and the adhesion-dependent signaling pathways governs the chemosensitivity of acute myeloid leukemia.
De Toni F, Racaud-Sultan C, Chicanne G, Mas VM, Cariven C, Mesange F, Salles JP, Demur C, Allouche M, Payrastre B, Manenti S, Ysebaert L. Oncogene. 2006 May 25;25(22):3113-22. doi: 10.1038/sj.onc.1209346. PMID: 16407823
Expression of focal adhesion kinase in acute myeloid leukemia is associated with enhanced blast migration, increased cellularity, and poor prognosis.
Recher C, Ysebaert L, Beyne-Rauzy O, Mansat-De Mas V, Ruidavets JB, Cariven P, Demur C, Payrastre B, Laurent G, Racaud-Sultan C. Cancer Res. 2004 May 1;64(9):3191-7. doi: 10.1158/0008-5472.can-03-3005. PMID: 15126359
Differential regulation of phosphoinositide metabolism by alphaVbeta3 and alphaVbeta5 integrins upon smooth muscle cell migration.
Paulhe F, Racaud-Sultan C, Ragab A, Albiges-Rizo C, Chap H, Iberg N, Morand O, Perret B. J Biol Chem. 2001 Nov 9;276(45):41832-40. doi: 10.1074/jbc.M105459200. Epub 2001 Sep 10. PMID: 11551924 Free article.
Lipid products of phosphoinositide 3-kinase and phosphatidylinositol 4',5'-bisphosphate are both required for ADP-dependent platelet spreading.
Heraud JM, Racaud-Sultan C, Gironcel D, Albigès-Rizo C, Giacomini T, Roques S, Martel V, Breton-Douillon M, Perret B, Chap H. J Biol Chem. 1998 Jul 10;273(28):17817-23. doi: 10.1074/jbc.273.28.17817. PMID: 9651384 Free article.
Sultan, C., Plantavid, M., Bachelot, C., Grondin, P., Breton, M., Mauco, G., Lévy-Toledano, S., Caen, J. P., and Chap, H. Involvement of platelet glycoprotein IIb-IIIa (aIIbb3-integrin) in thrombin-induced synthesis of phosphatidylinositol 3,4-bisphosphate. J. Biol. Chem. 1991, 266, 23554-23557.
Yan J, Racaud-Sultan C, Pezier T, Edir A, Rolland C, Claverie C, Burlaud-Gaillard J, Olivier M, Velge P, Lacroix-Lamandé S, Vergnolle N, Wiedemann A. Sci Rep. 2024 Jul 2;14(1):15160.
Daphnanes diterpenes from the latex of Hura crepitans L. and their PKCζ-dependent anti-proliferative activity on colorectal cancer cells.
Crossay E, Jullian V, Trinel M, Sagnat D, Hamel D, Groppi E, Rolland C, Stigliani JL, Mejia K, Cabanillas BJ, Alric L, Buscail E, El Kalamouni C, Mavingui P, Deraison C, Racaud-Sultan C, Fabre N. Bioorg Med Chem. 2023 Jul 15;90:117366.
Prenatal stress induces changes in PAR2- and M3-dependent regulation of colon primitive cells.
Berger M, Guiraud L, Dumas A, Sagnat D, Payros G, Rolland C, Vergnolle N, Deraison C, Cenac N, Racaud-Sultan C. Am J Physiol Gastrointest Liver Physiol. 2022 Dec 1;323(6):G609-G626
GSK3β, a Master Kinase in the Regulation of Adult Stem Cell Behavior.
Racaud-Sultan C, Vergnolle N. Cells. 2021 Jan 24;10(2):225. doi: 10.3390/cells10020225. PMID: 33498808 Free PMC article. Review.
Daphnanes diterpenes from the latex of Hura crepitans L. And activity against human colorectal cancer cells Caco-2.
Trinel M, Le Lamer AC, Jullian V, Jacquemin D, Graton J, Cristofoli V, Crossay E, Yassine M, Rolland C, Vergnolle N, Mejia K, Joel Cabanillas B, Racaud-Sultan C, Fabre N. Bioorg Chem. 2020 Oct;103:104132. doi: 10.1016/j.bioorg.2020.104132. Epub 2020 Jul 23. PMID: 32768743
Characterization of Human Colon Organoids From Inflammatory Bowel Disease Patients.
d'Aldebert E, Quaranta M, Sébert M, Bonnet D, Kirzin S, Portier G, Duffas JP, Chabot S, Lluel P, Allart S, Ferrand A, Alric L, Racaud-Sultan C, Mas E, Deraison C, Vergnolle N. Front Cell Dev Biol. 2020 Jun 4;8:363. doi: 10.3389/fcell.2020.00363. eCollection 2020. PMID: 32582690 Free PMC article.
Sexual dimorphism in PAR2-dependent regulation of primitive colonic cells.
Noguerol J, Roustan PJ, N'Taye M, Delcombel L, Rolland C, Guiraud L, Sagnat D, Edir A, Bonnart C, Denadai-Souza A, Deraison C, Vergnolle N, Racaud-Sultan C. Biol Sex Differ. 2019 Sep 6;10(1):47. doi: 10.1186/s13293-019-0262-6. PMID: 31492202 Free PMC article.
FAK alternative splice mRNA variants expression pattern in colorectal cancer.
Devaud C, Tilkin-Mariamé AF, Vignolle-Vidoni A, Souleres P, Denadai-Souza A, Rolland C, Duthoit C, Blanpied C, Chabot S, Bouillé P, Lluel P, Vergnolle N, Racaud-Sultan C, Ferrand A. Int J Cancer. 2019 Jul 15;145(2):494-502. doi: 10.1002/ijc.32120. Epub 2019 Feb 14. PMID: 30628725 Free PMC article.
Sustainable Positive Response to Sirolimus in Juvenile Polyposis of Infancy.
Quaranta M, Laborde N, Ferrand A, Danjoux M, Vergnolle N, Barreau F, Racaud-Sultan C, Mas E. J Pediatr Gastroenterol Nutr. 2019 Feb;68(2):e38-e40. doi: 10.1097/MPG.0000000000002179. PMID: 30334931 No abstract available.
Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a protease-activated receptor 1- and protease-activated receptor 4-dependent mechanism.
Sébert M, Denadai-Souza A, Quaranta M, Racaud-Sultan C, Chabot S, Lluel P, Monjotin N, Alric L, Portier G, Kirzin S, Bonnet D, Ferrand A, Vergnolle N. Br J Pharmacol. 2018 Sep;175(18):3656-3668. doi: 10.1111/bph.14430. Epub 2018 Aug 7. PMID: 29959891 Free PMC article.
[Progress in prostate cancer study: 3D cell culture enables the ex vivo reproduction of tumor characteristics].
Tostivint V, Racaud-Sultan C, Roumiguié M, Soulié M, Gamé X, Beauval JB. Presse Med. 2017 Oct;46(10):954-965. doi: 10.1016/j.lpm.2017.06.014. Epub 2017 Sep 28. PMID: 28967525 French.
Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells.
Nasri I, Chawech R, Girardi C, Mas E, Ferrand A, Vergnolle N, Fabre N, Mezghani-Jarraya R, Racaud-Sultan C. Pharm Biol. 2017 Dec;55(1):124-131. doi: 10.1080/13880209.2016.1230877. Epub 2016 Sep 22. PMID: 27925497 Free PMC article.
PAR2-dependent activation of GSK3β regulates the survival of colon stem/progenitor cells.
Nasri I, Bonnet D, Zwarycz B, d'Aldebert E, Khou S, Mezghani-Jarraya R, Quaranta M, Rolland C, Bonnart C, Mas E, Ferrand A, Cenac N, Magness S, Van Landeghem L, Vergnolle N, Racaud-Sultan C. Am J Physiol Gastrointest Liver Physiol. 2016 Aug 1;311(2):G221-36. doi: 10.1152/ajpgi.00328.2015. Epub 2016 Jun 16. PMID: 27313176 Free PMC article.
Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered Wnt signaling.
Despeaux M, Chicanne G, Rouer E, De Toni-Costes F, Bertrand J, Mansat-De Mas V, Vergnolle N, Eaves C, Payrastre B, Girault JA, Racaud-Sultan C. Stem Cells. 2012 Aug;30(8):1597-610. doi: 10.1002/stem.1157. PMID: 22714993
Sex differences in the GSK3β-mediated survival of adherent leukemic progenitors.
Bertrand J, Despeaux M, Joly S, Bourogaa E, Gallay N, Demur C, Bonnevialle P, Louache F, Maguer-Satta V, Vergnolle N, Payrastre B, Racaud-Sultan C. Oncogene. 2012 Feb 9;31(6):694-705. doi: 10.1038/onc.2011.258. Epub 2011 Jul 4. PMID: 21725365
Critical features of FAK-expressing AML bone marrow microenvironment through leukemia stem cell hijacking of mesenchymal stromal cells.
Despeaux M, Labat E, Gadelorge M, Prade N, Bertrand J, Demur C, Recher C, Bonnevialle P, Payrastre B, Bourin P, Racaud-Sultan C. Leukemia. 2011 Nov;25(11):1789-93. doi: 10.1038/leu.2011.145. Epub 2011 Jun 7. PMID: 21647157 No abstract available.
A New alpha5beta1 integrin-dependent survival pathway through GSK3beta activation in leukemic cells.
De Toni-Costes F, Despeaux M, Bertrand J, Bourogaa E, Ysebaert L, Payrastre B, Racaud-Sultan C. PLoS One. 2010 Mar 23;5(3):e9807. doi: 10.1371/journal.pone.0009807. PMID: 20352103 Free PMC article.
A crosstalk between the Wnt and the adhesion-dependent signaling pathways governs the chemosensitivity of acute myeloid leukemia.
De Toni F, Racaud-Sultan C, Chicanne G, Mas VM, Cariven C, Mesange F, Salles JP, Demur C, Allouche M, Payrastre B, Manenti S, Ysebaert L. Oncogene. 2006 May 25;25(22):3113-22. doi: 10.1038/sj.onc.1209346. PMID: 16407823
Expression of focal adhesion kinase in acute myeloid leukemia is associated with enhanced blast migration, increased cellularity, and poor prognosis.
Recher C, Ysebaert L, Beyne-Rauzy O, Mansat-De Mas V, Ruidavets JB, Cariven P, Demur C, Payrastre B, Laurent G, Racaud-Sultan C. Cancer Res. 2004 May 1;64(9):3191-7. doi: 10.1158/0008-5472.can-03-3005. PMID: 15126359
Differential regulation of phosphoinositide metabolism by alphaVbeta3 and alphaVbeta5 integrins upon smooth muscle cell migration.
Paulhe F, Racaud-Sultan C, Ragab A, Albiges-Rizo C, Chap H, Iberg N, Morand O, Perret B. J Biol Chem. 2001 Nov 9;276(45):41832-40. doi: 10.1074/jbc.M105459200. Epub 2001 Sep 10. PMID: 11551924 Free article.
Lipid products of phosphoinositide 3-kinase and phosphatidylinositol 4',5'-bisphosphate are both required for ADP-dependent platelet spreading.
Heraud JM, Racaud-Sultan C, Gironcel D, Albigès-Rizo C, Giacomini T, Roques S, Martel V, Breton-Douillon M, Perret B, Chap H. J Biol Chem. 1998 Jul 10;273(28):17817-23. doi: 10.1074/jbc.273.28.17817. PMID: 9651384 Free article.
Sultan, C., Plantavid, M., Bachelot, C., Grondin, P., Breton, M., Mauco, G., Lévy-Toledano, S., Caen, J. P., and Chap, H. Involvement of platelet glycoprotein IIb-IIIa (aIIbb3-integrin) in thrombin-induced synthesis of phosphatidylinositol 3,4-bisphosphate. J. Biol. Chem. 1991, 266, 23554-23557.
