Mini CV
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expertiseDNA extraction from mouse tail, intraperitoneal glucose/insulin/pyruvate tolerance test, retro-orbital cavity/tail-vein injection, mouse genotyping by Polymerase Chain Reaction (PCR), Enzyme-Linked Immunosorbent Assay (ELISA) and other biochemical assays, Western Blots, RNA/microRNA extraction and Real-Time PCR, Immunohistochemistry, mouse dissection, organ collection and cryopreservation, cell-culture, mice handling, management and establishment of mouse colonies, genetic background purification by crossing multiple generations. Establishment of axenic mice platform from scratch; microbial analyses for axenic mice status checking; preparation of bacterial suspension from mice/human stool; colonization/humanization of axenic mice; DNA extraction from mouse/human feces; metagenomic data management and analysis.
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Recherche/projet research/project
ENTEROBACTERIAL INFECTIONS AND DYSBIOSIS IN METABOLIC DISEASES, GUT INFLAMMATION AND CANCER