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EQUIPE 1 : PHYSIOPATHOLOGIE DE L’EPITHELIUM INTESTINAL
TEAM 1 : PATHOPHYSIOLOGY OF THE INTESTINAL EPITHELIUM |
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Mini CV
EDUCATION
2007-2011: Master of Science in Pharmacology and Toxicology (50/50)
Pharmacology - University of Naples "Federico II" – Naples, Italy.
2004-2007: PhD degree
Clinical and Experimental Medicine - Second University of Naples – Naples- Italy.
1999-2004: Master degree in Pharmacy (110/110 cum laude)
Experimental Pharmacology - University of Naples "Federico II" – Naples, Italy.
PROFESSIONAL EXPERIENCE
01/10/2015- 30/09/2018: Post-doctoral Fellow FWO (Research Foundation Flanders) - Laboratory for Enteric Neuroscience (LENS), TARGID - KU Leuven, Leuven, Belgium.
06/02/2015 – 06/08/2015: Visiting post-doc at INSERM U1043, Team "Mediators of Inflammation and Pain" Nathalie Vergnolle's group - CPTP Toulouse, France.
01/10/2012 - 30/09/2015: Post-doctoral Fellow FWO (Research Foundation Flanders) - Laboratory for Enteric Neuroscience (LENS), TARGID - KU Leuven, Leuven, Belgium.
01/10/2010 – 01/10/2012: Post-doctoral fellow KU Leuven - Laboratory for Enteric Neuroscience (LENS), TARGID - KU Leuven, Leuven, Belgium.
01/08/2008 - 01/08/2010: Post-doctoral fellow - Clinical and Experimental Medicine - University of Naples "Federico II" – Naples, Italy.
2007-2011: Master of Science in Pharmacology and Toxicology (50/50)
Pharmacology - University of Naples "Federico II" – Naples, Italy.
2004-2007: PhD degree
Clinical and Experimental Medicine - Second University of Naples – Naples- Italy.
1999-2004: Master degree in Pharmacy (110/110 cum laude)
Experimental Pharmacology - University of Naples "Federico II" – Naples, Italy.
PROFESSIONAL EXPERIENCE
01/10/2015- 30/09/2018: Post-doctoral Fellow FWO (Research Foundation Flanders) - Laboratory for Enteric Neuroscience (LENS), TARGID - KU Leuven, Leuven, Belgium.
06/02/2015 – 06/08/2015: Visiting post-doc at INSERM U1043, Team "Mediators of Inflammation and Pain" Nathalie Vergnolle's group - CPTP Toulouse, France.
01/10/2012 - 30/09/2015: Post-doctoral Fellow FWO (Research Foundation Flanders) - Laboratory for Enteric Neuroscience (LENS), TARGID - KU Leuven, Leuven, Belgium.
01/10/2010 – 01/10/2012: Post-doctoral fellow KU Leuven - Laboratory for Enteric Neuroscience (LENS), TARGID - KU Leuven, Leuven, Belgium.
01/08/2008 - 01/08/2010: Post-doctoral fellow - Clinical and Experimental Medicine - University of Naples "Federico II" – Naples, Italy.
expertise
- Isolation of ganglia and glial cells from human intestinal tissue (surgical samples or routine biopsies)
- Isolation of ganglia and glial cells from mouse/guinea pig intestinal tissue
- Tissue culture
- In vitro and in vivo techniques to asses intestinal motility
- Optical imaging technique (calcium imaging, mitochondria imaging) in live tissue/cells
- Microscopy (confocal, 2-photon, spinning disk)
Recherche/projet research/project
Rôle des protéases dans le système nerveux entérique humain
Characterization of protease signaling in the human enteric nervous system in health and disease
Since the Master Thesis (2002-2004, University “Federico II”, Naples, Italy), my research has focused on Enteric Neuroscience. I started to study gastrointestinal function in animals using both in vitro and in vivo models. During the PhD and the first post-doc (Naples, Italy), I focused my research on human intestinal diseases, with focus on inflammatory diseases. This period was really lucrative, since I set up a technique to isolate primary glial cells from human tissue. In 2010, to further improve my scientific knowledge and technical skills (optical imaging and hard-core microscopy), I moved to Belgium, at KU Leuven, where we successfully set up a method to record from neuronal/glia cells activity in human samples. In 2012 I got 3-years fellowship, which has been renewed for 3 years more by the Flemish government (FWO), to study more in detail the functioning of human enteric nervous system in health and disease. I was awarded with a travel grant to spend 6 months (February-August 2015) at the INSERM under the supervision of Dr. Nathalie Vergnolle and nowadays I’m spending a second period in her lab to further decipher the protease signaling in the human enteric nervous system. In parallel, I’m the Principal Investigator of a granted research project (CREA – @ KULeuven) aimed at maintaining in culture human nerve tissue.
Characterization of protease signaling in the human enteric nervous system in health and disease
Since the Master Thesis (2002-2004, University “Federico II”, Naples, Italy), my research has focused on Enteric Neuroscience. I started to study gastrointestinal function in animals using both in vitro and in vivo models. During the PhD and the first post-doc (Naples, Italy), I focused my research on human intestinal diseases, with focus on inflammatory diseases. This period was really lucrative, since I set up a technique to isolate primary glial cells from human tissue. In 2010, to further improve my scientific knowledge and technical skills (optical imaging and hard-core microscopy), I moved to Belgium, at KU Leuven, where we successfully set up a method to record from neuronal/glia cells activity in human samples. In 2012 I got 3-years fellowship, which has been renewed for 3 years more by the Flemish government (FWO), to study more in detail the functioning of human enteric nervous system in health and disease. I was awarded with a travel grant to spend 6 months (February-August 2015) at the INSERM under the supervision of Dr. Nathalie Vergnolle and nowadays I’m spending a second period in her lab to further decipher the protease signaling in the human enteric nervous system. In parallel, I’m the Principal Investigator of a granted research project (CREA – @ KULeuven) aimed at maintaining in culture human nerve tissue.
Production scientifique scientific production
1. Esposito G, Gigli S, Seguella L, Nobile N, d'Alessandro A, Pesce M, Capoccia E, Steardo L, Cirillo C, Cuomo R, Sarnelli G. Rifaximin, a non-absorbable antibiotic, inhibits the release of pro-angiogenic mediators in colon cancer cells through a pregnane X receptor-dependent pathway. Int J Oncol 2016.
2. Fournel A, Marlin A, Abot A, Pasquio C, Cirillo C, Cani P, Knauf C. Glucosensing in the gastrointestinal tract: Impact on glucose metabolism. Am J Physiol: GI & Liver 2016.
3. Esposito G, Sarnelli G, Capoccia E, Cirillo C, Pesce M, Lu J, Calí G, Cuomo R, Steardo L. Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration. Sci Rep 2016.
4. Sarnelli G, Gigli S, Capoccia E, Iuvone T, Cirillo C, Seguella L, Nobile N, D'Alessandro A, Pesce M, Steardo L, Cuomo R, Esposito G. Palmitoylethanolamide exerts antiproliferative effect and downregulates VEGF signaling in Caco-2 human colon carcinoma cell line through a selective PPAR-α-dependent inhibition of Akt/mTOR pathway. Phytother Res 2016.
5. Wouters MM, Balemans D, Van Wanrooy S, Dooley J, Cibert-Goton V, Alpizar YA, Valdez-Morales EE, Nasser Y, Van Veldhoven PP, Vanbrabant W, Van der Merwe S, Mols R, Ghesquière B, Cirillo C, et al. Histamine Receptor H1-mediated Sensitization of TRPV1 Mediates Visceral Hypersensitivity and Symptoms in Patients With Irritable Bowel Syndrome. Gastroenterology 2016.
6. Capoccia E, Cirillo C, Gigli S, Pesce M, D’Alessandro A, Cuomo R, Sarnelli G, Steardo L and Esposito G. Enteric glia: A new player in inflammatory bowel diseases. Int J Immunopathol Pharmacol. 2015.
7. Cirillo C, Bessissow T, Desmet AS, Vanheel H, Tack J, Vanden Berghe P. Evidence for neuronal dysfunction and structural changes in submucous ganglia of patients with functional dyspepsia. Am J Gastroenterol. 2015
8. Cirillo C, Capasso R. Constipation and botanical medicines: an overview. Phytother Res 2015
9. Cirillo C, Capoccia E, Iuvone T, Cuomo R, Sarnelli G, Steardo L, Esposito G. S100B inhibitor pentamidine attenuates reactive gliosis and reduces neuronal loss in a mouse model of Alzheimer’s disease. BioMed Res Int. 2015.
10. Capoccia E, Cirillo C, et al. S100B-p53 disengagement by pentamidine mediates apoptosis and inhibits cellular migration through aquaporin-4 and metalloproteinase-2 inhibition in C6 glioma cells. Oncology Letters 2014.
11. Herdewyn S, Cirillo C, Van Den Bosch L, Robberecht W, Vanden Berghe P, Van Damme P. Prevention of intestinal obstruction reveals progressive neurodegeneration in mutant TDP-43(A315T) mice. Mol Neurodegener. 2014;9(1):24. doi: 10.1186/1750-1326-9-24.
12. Desmet AS, Cirillo C, Vanden Berghe P. Distinct subcellular localization of the neuronal marker HuC/D reveals hypoxia-induced damage in enteric neurons. Neurogastroenterol Motil. 2014 May 25. doi: 10.1111/nmo.12371.
13. Rocha N, Reis H, Vanden Berghe P and Cirillo C. Behavioral changes in Parkinson’s disease: a role for inflammation and immunomodulation? Neuroimmunomodulation. 2014;21(2-3):88-94. doi: 10.1159/000356531.
14. Boesmans W, Martens M, Weltens N, Hao MM, Tack J, Cirillo C and Vanden Berghe P. Imaging neuron-glia interactions in the enteric nervous system. Frontiers in Cellular Neuroscience. 2013 Oct 21;7:183. doi: 10.3389/fncel.2013.00183.
15. Turco F, Sarnelli G, Cirillo C, Palumbo I, De Giorgi F, D'Alessandro A, Cammarota M, Giuliano MT, Cuomo R. Enteroglial-derived S100B protein integrates bacteria-induced Toll-like receptor signalling in human enteric glial cells. Gut 2013 doi:10.1136/gutjnl-2012-302090.
16. Boesmans W, Cirillo C, Van den Abbeel V, Van den Haute C, Depoortere I, Tack J, Vanden Berghe P. Neurotransmitters involved in fast excitatory neurotransmission directly activate enteric glial cells. Neurogastroenterol Motil. 2013. doi: 10.1111/nmo.12065. PMID: 23279281
17. Esposito G, Capoccia E, Sarnelli G, Scuderi C, Cirillo C, Cuomo R, Steardo L. The antiprotozoal drug pentamidine ameliorates experimentally induced acute colitis in mice. J Neuroinflammation. 2012 Dec 23;9(1):277. PMID: 23259641
18. De Giorgi F, Sarnelli G, Cirillo C, Savino IG, Turco F, Nardone G, Rocco A, Cuomo R. Increased severity of dyspeptic symptoms related to mental stress is associated with sympathetic hyperactivity and enhanced endocrine response in patients with postprandial distress syndrome. Neurogastroenterol Motil 2012 doi: 10.1111/nmo.12004.
19. Esposito G, Filippis DD, Cirillo C, Iuvone T, Capoccia E, Scuderi C, Steardo A, Cuomo R, Steardo L. Cannabidiol in Inflammatory Bowel Diseases: A Brief Overview. Phytother Res. 2012 doi: 10.1002/ptr.4781.
20. Cirillo C, Tack J, Vanden Berghe P. Nerve activity recordings in routine human intestinal biopsies. Gut. 2013 Feb;62(2):227-35. doi: 10.1136/gutjnl-2011-301777.
21. Cirillo C, Vanden Berghe P, Tack J. Role of serotonin in gastrointestinal physiology and pathology. Minerva Endocrinol. 2011 Dec;36(4):311-24.
22. De Filippis D, Esposito G, Cirillo C, et al. Cannabidiol reduces intestinal inflammation through the control of neuroimmune axis. PlosONE 2011;6(12):e28159.
23. Cuomo R, Savarese MF, Sarnelli G, Nicolai E, Aragri A, Cirillo C, Vozzella L, Zito FP, Verlezza V, Efficie E, Buyckx M. The role of a pre-load beverage on gastric volume and food intake: comparison between non-caloric carbonated and non-carbonated beverage. Nutr J. 2011 Oct 14;10(1):114.
24. Cirillo C, Sarnelli G, Turco F, Mango A, Grosso M, Aprea G, Masone S, Cuomo R. Proinflammatory stimuli activates human-derived enteroglial cells and induces autocrine nitric oxide production. Neurogastroenterol Motil. 2011 Sep;23(9):e372-82.
25. Cirillo C, Sarnelli G, Esposito G, Turco F, Steardo L, Cuomo R. S100B protein in the gut: the evidence for enteroglial-sustained intestinal inflammation. World Journal of Gastroenterology 2011 Mar 14;17(10):1261-6.
26. Cirillo C, Sarnelli G, Esposito G, Grosso M, Petruzzelli R, Izzo P, Calì G, D'Armiento FP, Rocco A, Nardone G, Iuvone T, Steardo L, Cuomo R. Increased mucosal nitric oxide production in ulcerative colitis is mediated in part by the enteroglial-derived S100B protein. NeurogastroenterolMotil. 2009;21;1209-1219.
27. Cuomo R, Savarese MF, Sarnelli G, Vollono G, Rocco A, Coccoli P, Cirillo C, Asciore L, Nardone G, Buyckx M. Sweetened carbonated drinks do not alter upper digestive tract physiology in healthy subjects. NeurogastroenterolMotil. 2008;20:780-789.
28. Esposito G, Cirillo C, Sarnelli G, De Filippis D, D'Armiento FP, Rocco A, Nardone G, Petruzzelli R, Grosso M, Izzo P, Iuvone T, Cuomo R. Enteric glial-derived s100b protein stimulates nitric oxide production in celiac disease. Gastroenterology 2007;133:918-925.
29. Esposito G, De Filippis D, Cirillo C, Sarnelli G, Cuomo R, Iuvone T. The astroglial-derived S100 Beta protein stimulates the expression of nitric oxide synthase in rodent macrophages through p38 MAP kinase activation. Life Sciences 2006;78:2707-2715.
2. Fournel A, Marlin A, Abot A, Pasquio C, Cirillo C, Cani P, Knauf C. Glucosensing in the gastrointestinal tract: Impact on glucose metabolism. Am J Physiol: GI & Liver 2016.
3. Esposito G, Sarnelli G, Capoccia E, Cirillo C, Pesce M, Lu J, Calí G, Cuomo R, Steardo L. Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration. Sci Rep 2016.
4. Sarnelli G, Gigli S, Capoccia E, Iuvone T, Cirillo C, Seguella L, Nobile N, D'Alessandro A, Pesce M, Steardo L, Cuomo R, Esposito G. Palmitoylethanolamide exerts antiproliferative effect and downregulates VEGF signaling in Caco-2 human colon carcinoma cell line through a selective PPAR-α-dependent inhibition of Akt/mTOR pathway. Phytother Res 2016.
5. Wouters MM, Balemans D, Van Wanrooy S, Dooley J, Cibert-Goton V, Alpizar YA, Valdez-Morales EE, Nasser Y, Van Veldhoven PP, Vanbrabant W, Van der Merwe S, Mols R, Ghesquière B, Cirillo C, et al. Histamine Receptor H1-mediated Sensitization of TRPV1 Mediates Visceral Hypersensitivity and Symptoms in Patients With Irritable Bowel Syndrome. Gastroenterology 2016.
6. Capoccia E, Cirillo C, Gigli S, Pesce M, D’Alessandro A, Cuomo R, Sarnelli G, Steardo L and Esposito G. Enteric glia: A new player in inflammatory bowel diseases. Int J Immunopathol Pharmacol. 2015.
7. Cirillo C, Bessissow T, Desmet AS, Vanheel H, Tack J, Vanden Berghe P. Evidence for neuronal dysfunction and structural changes in submucous ganglia of patients with functional dyspepsia. Am J Gastroenterol. 2015
8. Cirillo C, Capasso R. Constipation and botanical medicines: an overview. Phytother Res 2015
9. Cirillo C, Capoccia E, Iuvone T, Cuomo R, Sarnelli G, Steardo L, Esposito G. S100B inhibitor pentamidine attenuates reactive gliosis and reduces neuronal loss in a mouse model of Alzheimer’s disease. BioMed Res Int. 2015.
10. Capoccia E, Cirillo C, et al. S100B-p53 disengagement by pentamidine mediates apoptosis and inhibits cellular migration through aquaporin-4 and metalloproteinase-2 inhibition in C6 glioma cells. Oncology Letters 2014.
11. Herdewyn S, Cirillo C, Van Den Bosch L, Robberecht W, Vanden Berghe P, Van Damme P. Prevention of intestinal obstruction reveals progressive neurodegeneration in mutant TDP-43(A315T) mice. Mol Neurodegener. 2014;9(1):24. doi: 10.1186/1750-1326-9-24.
12. Desmet AS, Cirillo C, Vanden Berghe P. Distinct subcellular localization of the neuronal marker HuC/D reveals hypoxia-induced damage in enteric neurons. Neurogastroenterol Motil. 2014 May 25. doi: 10.1111/nmo.12371.
13. Rocha N, Reis H, Vanden Berghe P and Cirillo C. Behavioral changes in Parkinson’s disease: a role for inflammation and immunomodulation? Neuroimmunomodulation. 2014;21(2-3):88-94. doi: 10.1159/000356531.
14. Boesmans W, Martens M, Weltens N, Hao MM, Tack J, Cirillo C and Vanden Berghe P. Imaging neuron-glia interactions in the enteric nervous system. Frontiers in Cellular Neuroscience. 2013 Oct 21;7:183. doi: 10.3389/fncel.2013.00183.
15. Turco F, Sarnelli G, Cirillo C, Palumbo I, De Giorgi F, D'Alessandro A, Cammarota M, Giuliano MT, Cuomo R. Enteroglial-derived S100B protein integrates bacteria-induced Toll-like receptor signalling in human enteric glial cells. Gut 2013 doi:10.1136/gutjnl-2012-302090.
16. Boesmans W, Cirillo C, Van den Abbeel V, Van den Haute C, Depoortere I, Tack J, Vanden Berghe P. Neurotransmitters involved in fast excitatory neurotransmission directly activate enteric glial cells. Neurogastroenterol Motil. 2013. doi: 10.1111/nmo.12065. PMID: 23279281
17. Esposito G, Capoccia E, Sarnelli G, Scuderi C, Cirillo C, Cuomo R, Steardo L. The antiprotozoal drug pentamidine ameliorates experimentally induced acute colitis in mice. J Neuroinflammation. 2012 Dec 23;9(1):277. PMID: 23259641
18. De Giorgi F, Sarnelli G, Cirillo C, Savino IG, Turco F, Nardone G, Rocco A, Cuomo R. Increased severity of dyspeptic symptoms related to mental stress is associated with sympathetic hyperactivity and enhanced endocrine response in patients with postprandial distress syndrome. Neurogastroenterol Motil 2012 doi: 10.1111/nmo.12004.
19. Esposito G, Filippis DD, Cirillo C, Iuvone T, Capoccia E, Scuderi C, Steardo A, Cuomo R, Steardo L. Cannabidiol in Inflammatory Bowel Diseases: A Brief Overview. Phytother Res. 2012 doi: 10.1002/ptr.4781.
20. Cirillo C, Tack J, Vanden Berghe P. Nerve activity recordings in routine human intestinal biopsies. Gut. 2013 Feb;62(2):227-35. doi: 10.1136/gutjnl-2011-301777.
21. Cirillo C, Vanden Berghe P, Tack J. Role of serotonin in gastrointestinal physiology and pathology. Minerva Endocrinol. 2011 Dec;36(4):311-24.
22. De Filippis D, Esposito G, Cirillo C, et al. Cannabidiol reduces intestinal inflammation through the control of neuroimmune axis. PlosONE 2011;6(12):e28159.
23. Cuomo R, Savarese MF, Sarnelli G, Nicolai E, Aragri A, Cirillo C, Vozzella L, Zito FP, Verlezza V, Efficie E, Buyckx M. The role of a pre-load beverage on gastric volume and food intake: comparison between non-caloric carbonated and non-carbonated beverage. Nutr J. 2011 Oct 14;10(1):114.
24. Cirillo C, Sarnelli G, Turco F, Mango A, Grosso M, Aprea G, Masone S, Cuomo R. Proinflammatory stimuli activates human-derived enteroglial cells and induces autocrine nitric oxide production. Neurogastroenterol Motil. 2011 Sep;23(9):e372-82.
25. Cirillo C, Sarnelli G, Esposito G, Turco F, Steardo L, Cuomo R. S100B protein in the gut: the evidence for enteroglial-sustained intestinal inflammation. World Journal of Gastroenterology 2011 Mar 14;17(10):1261-6.
26. Cirillo C, Sarnelli G, Esposito G, Grosso M, Petruzzelli R, Izzo P, Calì G, D'Armiento FP, Rocco A, Nardone G, Iuvone T, Steardo L, Cuomo R. Increased mucosal nitric oxide production in ulcerative colitis is mediated in part by the enteroglial-derived S100B protein. NeurogastroenterolMotil. 2009;21;1209-1219.
27. Cuomo R, Savarese MF, Sarnelli G, Vollono G, Rocco A, Coccoli P, Cirillo C, Asciore L, Nardone G, Buyckx M. Sweetened carbonated drinks do not alter upper digestive tract physiology in healthy subjects. NeurogastroenterolMotil. 2008;20:780-789.
28. Esposito G, Cirillo C, Sarnelli G, De Filippis D, D'Armiento FP, Rocco A, Nardone G, Petruzzelli R, Grosso M, Izzo P, Iuvone T, Cuomo R. Enteric glial-derived s100b protein stimulates nitric oxide production in celiac disease. Gastroenterology 2007;133:918-925.
29. Esposito G, De Filippis D, Cirillo C, Sarnelli G, Cuomo R, Iuvone T. The astroglial-derived S100 Beta protein stimulates the expression of nitric oxide synthase in rodent macrophages through p38 MAP kinase activation. Life Sciences 2006;78:2707-2715.
