groupe de recherche: environnement et l'épithélium intestinaL
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GROUPE DE RECHERCHE
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AUDREY FERRAND & FRÉDÉRICK BARREAUChefs de Groupe
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DESCRIPTION DU PROGRAMME DE RECHERCHE
L’épithélium intestinal représente une barrière fonctionnelle essentielle pour notre organisme en permettant l’absorption des nutriments, des sels et de l’eau tout en assurant une protection contre les agents pathogènes. Afin d’assurer ces fonctions, les cellules de la muqueuse intestinale se renouvellent tous les 5 jours, soumettant le tractus digestif à un stress important dans le contrôle de la prolifération, la différenciation et l’organisation cellulaire de l’épithélium intestinal. Le renouvellement intestinal est étroitement contrôlé et dépend notamment de l'organisation spatiale des signaux émanant des cellules mésenchymateuses de soutien, en particulier les fibroblastes gainant la crypte et soutenant l’épithélium intestinal. L’homéostasie, et donc l'intégrité de l’épithélium intestinal, est constamment mise à l’épreuve par les facteurs environnementaux ‘extérieurs’ provenant de la lumière intestinale (additifs alimentaires, composés nutritionnels, microbiote…) ou ‘intérieurs’ (stroma, prédisposition génétiques…). Les facteurs environnementaux favorisant et/ou aggravant l'inflammation colique ou affectant le processus de renouvellement des cellules épithéliales sont une source majeure de préoccupation. En effet, toute altération de l’homéostasie intestinale va intervenir dans la mise en places des maladies inflammatoires chroniques de l’intestin (MICI) et la transformation tumorale.
Les deux principales formes de MICI sont la maladie de Crohn (MC) et la rectocolite hémorragique (RCH). L’une des complications majeures des MICI est le cancer colorectal. D’autre part, le cancer colorectal sporadique (CRC) est la 3ème cause de mortalité par cancer dans les pays industrialisés.
Nos projets de recherche ont pour but d’étudier l’impact environnemental (‘extérieur’ ou ‘intérieur’) sur la physio(patho)logie de l’épithélium intestinal, le développement du processus inflammatoire et la carcinogénèse.
Les deux principales formes de MICI sont la maladie de Crohn (MC) et la rectocolite hémorragique (RCH). L’une des complications majeures des MICI est le cancer colorectal. D’autre part, le cancer colorectal sporadique (CRC) est la 3ème cause de mortalité par cancer dans les pays industrialisés.
Nos projets de recherche ont pour but d’étudier l’impact environnemental (‘extérieur’ ou ‘intérieur’) sur la physio(patho)logie de l’épithélium intestinal, le développement du processus inflammatoire et la carcinogénèse.
Membres
ANCIENS MEMBRES
modèles cellulaires et animaux / méthodes
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Modèles cellulaires:
Modèles animaux
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Méthodes:
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publications
Nod2 protects the gut from spreading experimental colitis to small intestine.
Al Nabhani Z, Berrebi D, Martinez-Vinson C, Montcuquet N, Madre C, Roy M, Ogier-Denis E, Dussaillant M, Cerf-Bensussan N, Zouali H, Daniel F, Barreau F, Hugot JP.J Crohns Colitis. 2019 Nov 30:jjz196. doi: 10.1093/ecco-jcc/jjz196
The interplay between genetic risk factors and proteolytic dysregulation in the pathophysiology of inflammatory bowel disease.
Solà Tapias N, Vergnolle N, Denadai-Souza A, Barreau F.J Crohns Colitis. 2020 Feb 22:jjaa033. doi: 10.1093/ecco-jcc/jjaa033
Toxicological impact of acute exposure to E171 food additive and TiO2 nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cells.
Dorier M, Tisseyre C, Dussert F, Béal D, Arnal ME, Douki T, Valdiglesias V, Laffon B, Fraga S, Brandão F, Herlin-Boime N, Barreau F, Rabilloud T, Carriere M.Mutat Res. 2019 Sep;845:402980. doi: 10.1016/j.mrgentox.2018.11.004.
Protease-Activated Receptors in the Intestine: Focus on Inflammation and Cancer.
Sébert M, Sola-Tapias N, Mas E, Barreau F, Ferrand A. Front Endocrinol (Lausanne). 2019 Oct 24;10:717. doi: 10.3389/fendo.2019.00717. eCollection 2019. Review. PubMed PMID: 31708870; PubMed Central PMCID: PMC6821688.
Latest Advances in Targeting the Tumor Microenvironment for Tumor Suppression.
Laplagne C, Domagala M, Le Naour A, Quemerais C, Hamel D, Fournié JJ, Couderc B, Bousquet C, Ferrand A, Poupot M.Int J Mol Sci. 2019 Sep 23;20(19). pii: E4719. doi: 10.3390/ijms20194719. Review. PubMed PMID: 31547627; PubMed Central PMCID: PMC6801830.Free PMC article. Review.
Toxicological impact of acute exposure to E171 food additive and TiO(2) nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cells.
Dorier M, Tisseyre C, Dussert F, Béal D, Arnal ME, Douki T, Valdiglesias V, Laffon B, Fraga S, Brandão F, Herlin-Boime N, Barreau F, Rabilloud T, Carriere M. Mutat Res. 2019 Sep;845:402980. doi: 10.1016/j.mrgentox.2018.11.004. Epub 2018 Nov 22. PubMed PMID: 31561898.
FAK alternative splice mRNA variants expression pattern in colorectal cancer.
Devaud C, Tilkin-Mariamé AF, Vignolle-Vidoni A, Souleres P, Denadai-Souza A, Rolland C, Duthoit C, Blanpied C, Chabot S, Bouillé P, Lluel P, Vergnolle N, Racaud-Sultan C, Ferrand A.Int J Cancer. 2019 Jul 15;145(2):494-502. doi: 10.1002/ijc.32120. Epub 2019 Feb 14.PMID: 30628725 Free PMC article.
Increasing uptake of silica nanoparticles with electroporation: from cellular characterization to potential applications
Phonesouk E, Lechevallier S, Ferrand A, Rols MP, Bezombes C, Verelst M, Golzio M. Materials (Basel). 2019 Jan 7;12(1). pii: E179. doi: 10.3390/ma12010179
NOD2 expression in intestinal epithelial cells protects toward the development of inflammation and associated carcinogenesis
Ferrand A, Al Nabhani Z, Solà Tapias N, Mas E, Hugot JP, Barreau F, Cellular and Molecular Gastroenterology and Hepatology, Cell Mol Gastroenterol Hepatol. 2019;7(2):357-369. doi: 10.1016/j.jcmgh.2018.10.009. Epub 2018 Oct 23. Review
Sustainable Positive Response to Sirolimus in Juvenile Polyposis of Infancy.
Quaranta M, Laborde N, Ferrand A, Danjoux M, Vergnolle N, Barreau F, Racaud-Sultan C, Mas E. J Pediatr Gastroenterol Nutr. 2018 Oct 16. doi: 10.1097/MPG.0000000000002179.PMID: 30334931
Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a PAR1- and PAR4-dependent mechanism.
Sébert M, Denadai-Souza A, Quaranta M, Racaud-Sultan C, Chabot S, Lluel P, Monjotin N, Alric L, Portier G, Kirzin S, Bonnet D, Ferrand A*, Vergnolle N*. (*: Co-last authors) Br J Pharmacol. 2018 Jun 30. doi: 10.1111/bph.14430. PMID: 29959891
Identification of an analgesic lipopeptide produced by the probiotic Escherichia coli strain Nissle 1917.
Pérez-Berezo T, Pujo J, Martin P, Le Faouder P, Galano JM, Guy A, Knauf C, Tabet JC, Tronnet S, Barreau F, Heuillet M, Dietrich G, Bertrand-Michel J, Durand T, Oswald E, Cenac N. Nat Commun. 2017 Nov 3;8(1):1314. doi: 10.1038/s41467-017-01403-9. PMID: 29101366 Free PMC Article Similar articles
Oral Administration of the Probiotic Strain Escherichia coli Nissle 1917 Reduces Susceptibility to Neuroinflammation and Repairs Experimental Autoimmune Encephalomyelitis-Induced Intestinal Barrier Dysfunction.
Secher T, Kassem S, Benamar M, Bernard I, Boury M, Barreau F, Oswald E, Saoudi A. Front Immunol. 2017 Sep 14;8:1096. doi: 10.3389/fimmu.2017.01096. eCollection 2017. PMID:28959254 Free PMC Article Similar articles
Complementary Roles of Nod2 in Hematopoietic and Nonhematopoietic Cells in Preventing Gut Barrier Dysfunction Dependent on MLCK Activity.
Al Nabhani Z, Montcuquet N, Roy M, Dussaillant M, Hugot JP, Barreau F. Inflamm Bowel Dis. 2017 Jul;23(7):1109-1119. doi: 10.1097/MIB.0000000000001135. PMID:28520587 Similar articles
Nod2: The intestinal gate keeper.
Al Nabhani Z, Dietrich G, Hugot JP, Barreau F. PLoS Pathog. 2017 Mar 2;13(3):e1006177. doi: 10.1371/journal.ppat.1006177. eCollection 2017 Mar. Review. PMID:28253332 Free PMC Article Similar articles
Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells.
Nasri I, Chawech R, Girardi C, Mas E, Ferrand A, Vergnolle N, Fabre N, Mezghani-Jarraya R, Racaud-Sultan C. Pharm Biol. 2017 Dec;55(1):124-131. Epub 2016 Sep 22. PMID:27925497 Similar articles
Mu and delta opioid receptor knockout mice show increased colonic sensitivity.
Reiss D, Ceredig RA, Secher T, Boué J, Barreau F, Dietrich G, Gavériaux-Ruff C. Eur J Pain. 2017 Apr;21(4):623-634. doi: 10.1002/ejp.965. Epub 2016 Oct 17. PMID:27748566 Similar articles
Al Nabhani Z, Berrebi D, Martinez-Vinson C, Montcuquet N, Madre C, Roy M, Ogier-Denis E, Dussaillant M, Cerf-Bensussan N, Zouali H, Daniel F, Barreau F, Hugot JP.J Crohns Colitis. 2019 Nov 30:jjz196. doi: 10.1093/ecco-jcc/jjz196
The interplay between genetic risk factors and proteolytic dysregulation in the pathophysiology of inflammatory bowel disease.
Solà Tapias N, Vergnolle N, Denadai-Souza A, Barreau F.J Crohns Colitis. 2020 Feb 22:jjaa033. doi: 10.1093/ecco-jcc/jjaa033
Toxicological impact of acute exposure to E171 food additive and TiO2 nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cells.
Dorier M, Tisseyre C, Dussert F, Béal D, Arnal ME, Douki T, Valdiglesias V, Laffon B, Fraga S, Brandão F, Herlin-Boime N, Barreau F, Rabilloud T, Carriere M.Mutat Res. 2019 Sep;845:402980. doi: 10.1016/j.mrgentox.2018.11.004.
Protease-Activated Receptors in the Intestine: Focus on Inflammation and Cancer.
Sébert M, Sola-Tapias N, Mas E, Barreau F, Ferrand A. Front Endocrinol (Lausanne). 2019 Oct 24;10:717. doi: 10.3389/fendo.2019.00717. eCollection 2019. Review. PubMed PMID: 31708870; PubMed Central PMCID: PMC6821688.
Latest Advances in Targeting the Tumor Microenvironment for Tumor Suppression.
Laplagne C, Domagala M, Le Naour A, Quemerais C, Hamel D, Fournié JJ, Couderc B, Bousquet C, Ferrand A, Poupot M.Int J Mol Sci. 2019 Sep 23;20(19). pii: E4719. doi: 10.3390/ijms20194719. Review. PubMed PMID: 31547627; PubMed Central PMCID: PMC6801830.Free PMC article. Review.
Toxicological impact of acute exposure to E171 food additive and TiO(2) nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cells.
Dorier M, Tisseyre C, Dussert F, Béal D, Arnal ME, Douki T, Valdiglesias V, Laffon B, Fraga S, Brandão F, Herlin-Boime N, Barreau F, Rabilloud T, Carriere M. Mutat Res. 2019 Sep;845:402980. doi: 10.1016/j.mrgentox.2018.11.004. Epub 2018 Nov 22. PubMed PMID: 31561898.
FAK alternative splice mRNA variants expression pattern in colorectal cancer.
Devaud C, Tilkin-Mariamé AF, Vignolle-Vidoni A, Souleres P, Denadai-Souza A, Rolland C, Duthoit C, Blanpied C, Chabot S, Bouillé P, Lluel P, Vergnolle N, Racaud-Sultan C, Ferrand A.Int J Cancer. 2019 Jul 15;145(2):494-502. doi: 10.1002/ijc.32120. Epub 2019 Feb 14.PMID: 30628725 Free PMC article.
Increasing uptake of silica nanoparticles with electroporation: from cellular characterization to potential applications
Phonesouk E, Lechevallier S, Ferrand A, Rols MP, Bezombes C, Verelst M, Golzio M. Materials (Basel). 2019 Jan 7;12(1). pii: E179. doi: 10.3390/ma12010179
NOD2 expression in intestinal epithelial cells protects toward the development of inflammation and associated carcinogenesis
Ferrand A, Al Nabhani Z, Solà Tapias N, Mas E, Hugot JP, Barreau F, Cellular and Molecular Gastroenterology and Hepatology, Cell Mol Gastroenterol Hepatol. 2019;7(2):357-369. doi: 10.1016/j.jcmgh.2018.10.009. Epub 2018 Oct 23. Review
Sustainable Positive Response to Sirolimus in Juvenile Polyposis of Infancy.
Quaranta M, Laborde N, Ferrand A, Danjoux M, Vergnolle N, Barreau F, Racaud-Sultan C, Mas E. J Pediatr Gastroenterol Nutr. 2018 Oct 16. doi: 10.1097/MPG.0000000000002179.PMID: 30334931
Thrombin modifies growth, proliferation and apoptosis of human colon organoids: a PAR1- and PAR4-dependent mechanism.
Sébert M, Denadai-Souza A, Quaranta M, Racaud-Sultan C, Chabot S, Lluel P, Monjotin N, Alric L, Portier G, Kirzin S, Bonnet D, Ferrand A*, Vergnolle N*. (*: Co-last authors) Br J Pharmacol. 2018 Jun 30. doi: 10.1111/bph.14430. PMID: 29959891
Identification of an analgesic lipopeptide produced by the probiotic Escherichia coli strain Nissle 1917.
Pérez-Berezo T, Pujo J, Martin P, Le Faouder P, Galano JM, Guy A, Knauf C, Tabet JC, Tronnet S, Barreau F, Heuillet M, Dietrich G, Bertrand-Michel J, Durand T, Oswald E, Cenac N. Nat Commun. 2017 Nov 3;8(1):1314. doi: 10.1038/s41467-017-01403-9. PMID: 29101366 Free PMC Article Similar articles
Oral Administration of the Probiotic Strain Escherichia coli Nissle 1917 Reduces Susceptibility to Neuroinflammation and Repairs Experimental Autoimmune Encephalomyelitis-Induced Intestinal Barrier Dysfunction.
Secher T, Kassem S, Benamar M, Bernard I, Boury M, Barreau F, Oswald E, Saoudi A. Front Immunol. 2017 Sep 14;8:1096. doi: 10.3389/fimmu.2017.01096. eCollection 2017. PMID:28959254 Free PMC Article Similar articles
Complementary Roles of Nod2 in Hematopoietic and Nonhematopoietic Cells in Preventing Gut Barrier Dysfunction Dependent on MLCK Activity.
Al Nabhani Z, Montcuquet N, Roy M, Dussaillant M, Hugot JP, Barreau F. Inflamm Bowel Dis. 2017 Jul;23(7):1109-1119. doi: 10.1097/MIB.0000000000001135. PMID:28520587 Similar articles
Nod2: The intestinal gate keeper.
Al Nabhani Z, Dietrich G, Hugot JP, Barreau F. PLoS Pathog. 2017 Mar 2;13(3):e1006177. doi: 10.1371/journal.ppat.1006177. eCollection 2017 Mar. Review. PMID:28253332 Free PMC Article Similar articles
Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells.
Nasri I, Chawech R, Girardi C, Mas E, Ferrand A, Vergnolle N, Fabre N, Mezghani-Jarraya R, Racaud-Sultan C. Pharm Biol. 2017 Dec;55(1):124-131. Epub 2016 Sep 22. PMID:27925497 Similar articles
Mu and delta opioid receptor knockout mice show increased colonic sensitivity.
Reiss D, Ceredig RA, Secher T, Boué J, Barreau F, Dietrich G, Gavériaux-Ruff C. Eur J Pain. 2017 Apr;21(4):623-634. doi: 10.1002/ejp.965. Epub 2016 Oct 17. PMID:27748566 Similar articles
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