- 2015- 2018 PhD degre Pathophysiology - Université Paul Sabatier - Toulouse, France.
- 2013-2014 Master degree Biomedical Research - Universitat Pompeu Fabra - Barcelona, Spain
- 2008-2013 BSc degree Biomedical Science - Universitat de Barcelona - Barcelona, Spain
- 2018-Present Post-doctoral fellow - Digestive Health Research Institut (IRSD, INSERM) -Toulouse, France.
- 2015–2018 PhD candidate - Digestive Health Research Institut (IRSD, INSERM) - Toulouse, France.
- 2014 Master Internship - Hôpital Bichat/ Institut Pasteur - Paris, France.
- 2012 - 2013 Bachelor internship - Nederlands Kanker Instituut (NKI) - Amsterdam, The Netherlands.
- Cell culture / organoid culture
- Protease quantification
- Permeability test (Dextran-FITC)
- Signal transduction study (proteomic and transcriptomic)
- Microscopy (confocal)
- Activated-Based Probe assay.
Crohn's disease (CD) and Ulcerative colitis (UC) are two forms of Inflammatory Bowel Disease (IBD), a chronic inflammatory pathology affecting the digestive tract. Although the molecular mechanisms of IBD are poorly understood, recent data suggest that IBD occurs in genetically predisposed individuals developing an abnormal immune response to intestinal microbes after, being exposed to specific environmental triggers. The strongest genetic associations have highlighted three main pathways altered in IBD including bacterial sensing (NOD2, CD), autophagy (ATG16L1 and IRGM, CD) and endoplasmic reticulum stress (ER-Stress) (XBP1, UC). In this project, we aimed at better understanding whether the three main pathways involved in IBD (Nod2, autophagy, ER-stress) could be linked to an epithelial release of trypsin and reciprocally, if epithelial trypsin is able to induce or modulate these three IBD pathways.
Production scientifique scientific production
Alexandre Denadai-Souza, Chrystelle Bonnart, Núria Solà Tapias et al. Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease. Scientific Report.