MINI CV
Après avoir obtenu un doctorat dans le laboratoire du Dr Mamadou Daffé à l'Institut de Pharmacologie et de Biologie Structurale en 2009 à Toulouse, France, j'ai rejoint le laboratoire du Dr Carl Nathan à Weill Cornell Medicine à New York, USA, au sein duquel j’ai été successivement chercheur postdoctoral (2009-2014), instructeur (2014-2017) et professeur (2017-2018). Cette expérience a continué d’aiguiser mon intérêt pour la résolution de problèmes biologiques par des approches multidisciplinaires, y compris la microbiologie, la biochimie, la génétique, la biologie structurale, la « time-lapse » microscopie et la microscopie super-résolution. En 2019, j'ai rejoint le département des maladies infectieuses à l'Imperial College de Londres, au sein duquel mon laboratoire étudiait les mécanismes par lesquels les bactéries, notamment les mycobactéries et E. coli uropathogènes, survivent transitoirement à une concentration létale d’un antibiotique. Depuis janvier 2024, je poursuis cet axe de recherche à l'Institut national de la santé et de la recherche médicale/Institut de recherche en santé digestive.
After completing my PhD in the laboratory of Dr. Mamadou Daffé at the Institute of Pharmacology and Structural Biology in 2009 in Toulouse, France, I joined the laboratory of Dr. Carl Nathan at Weill Cornell Medicine in New York, USA, as a postdoctoral fellow (2009-2014), an instructor (2014-2017) and an assistant professor (2017-2018). This experience spurred a continued interest in approaching research through multi-disciplinary approaches—spanning microbiology, biochemistry, genetics, structural biology, time-lapse and super-resolution fluorescence photomicroscopy. In 2019, I joined Imperial College London as a Lecturer in Molecular Microbiology in the department of Infectious Disease where my laboratory studied the mechanisms by which bacteria, including mycobacteria and uropathogenic E. coli, survive for a prolonged time upon exposure to otherwise lethal concentration of an antibiotic. I am continuing this line of research at the French National Institute of Health and Medical Research/ Research Institute of Digestive Health since January 2024.
Projets de recherche / research projects :
BACTÉRIES PATHOGÈNES ET RÉSISTANCE AUX ANTIBIOTIQUES
PATHOGENIC BACTERIA AND ANTIBIOTIC RESITANCE
PATHOGENIC BACTERIA AND ANTIBIOTIC RESITANCE
production scientifique / scientific production
Schrader SM, Botella H, Vaubourgeix J. Re-framing antimicrobial resistance as a continuous spectrum of manifestations. Curr Opin in Microbiol. 2023 Jan 4;72:102259.
Schrader SM, Botella H, Jansen R, Ehrt S, Rhee K, Nathan C#, Vaubourgeix J#. Multiform antimicrobial resistance from a metabolic mutation. Science Advances. 2021 Aug 27;7(35):eabh2037. Lead corresponding author.
Harnagel A, Lopez Quezada L, Park SW, Baranowski C, Kieser K, Jiang X, Roberts J, Vaubourgeix J, Yang A, Nelson B, Fay Allison, Rubin E, Ehrt S, Nathan C, Lupoli TJ. Nonredundant functions of Mycobacterium tuberculosis chaperones promote survival under stress. Mol Microbiol. 2021 Feb;115(2):272-289.
Wang R, Kreutzfeldt K, Botella H, Vaubourgeix J, Schnappinger D, Ehrt S. Persistent Mycobacterium tuberculosis infection in mice requires PerM for successful cell division. Elife. 2019 Nov;8:e49570.
McKenney P, Yan J, Vaubourgeix J, Becattini S, Lampen N, Motzer A, Larson PJ, Dannoui D, Fujisawa S, Xavier JB, Pamer EG. Intestinal bile acids induce a morphotype switch in vancomycin resistant Enterococcus that facilitates intestinal colonization. Cell Host Microbe. 2019 May 8;25(5):695-705.
Ballinger E, Mosior J, Hartman T, Burns-Huang K, Gold B, Morris R, Goullieux L, Blanc I, Vaubourgeix J, Lagrange S, Fraisse L, Sans S, Couturier C, Bacqué E, Rhee K, Scarry SM, Aubé J, Yang G, Ouerfelli O, Schnappinger D, Ioerger TR, Engelhart CA, McConnell JA, McAulay K, Fay A, Roubert C, Sacchettini J, Nathan C. Opposing reactions in coenzyme A metabolism sensitize Mycobacterium tuberculosis to enzyme inhibition. Science. 2019 Feb 1;363(6426).
Botella H, Yang G, Ouerfelli O, Ehrt S, Nathan C, Vaubourgeix J. Distinct spatio-temporal dynamics in peptidoglycan synthesis between M. smegmatis and M. tuberculosis. mBio. 2017 Sep 12;8(5).
Botella H, Vaubourgeix J, Song N, Xu W, Makinoshima H, Lee MH, Glickman MS and Ehrt S. Mycobacterium tuberculosis periplasmic protease MarP activates the peptidoglycan hydrolase RipA during acid stress. EMBO J. 2017 Feb 15;36(4):536-548.
Botella L, Vaubourgeix J, Livny J, Schnappinger D. Depleting Mycobacterium tuberculosis of the transcription termination factor Rho causes pervasive transcription and rapid death. Nature Communications. 2017 March 28; 8:14731.
Goodsmith N, Guo XV, Vandal OH, Vaubourgeix J, Wang R, Botella H, Song S, Bhatt K, Liba A, Salgame P, Schnappinger D, Ehrt S. Disruption of an M. tuberculosis membrane protein causes a magnesium-dependent cell division defect and failure to persist in mice. PLoS Pathogens. 2015 Feb 6;11(2):e1004645.
Vaubourgeix J, Lin G, Dhar N, Chenouard N, Jiang X, Botella H, Lupoli T, Mariani O, Yang G, Ouerfelli O, Unser M, Schnappinger D, McKinney J, Nathan C. Stressed mycobacteria use the chaperone ClpB to sequester irreversibly oxidized proteins asymmetrically within and between cells. Cell Host Microbe. 2015 Feb 11;17(2):178-90.
Lin G, Chidawanyika T, Tsu C, Warrier T, Vaubourgeix J, Blackburn C, Gigstad K, Sintchak M, Dick L, Nathan C. N,C-Capped dipeptides with selectivity for mycobacterial proteosome over human proteasomes: role of S3 and S1 binding pockets. J Am Chem Soc. 2013;135(27):9968-71.
Grzegorzewicz AE, Korduláková J, Jones V, Born SE, Belardinelli JM, Vaquié A, Gundi VA, Madacki J, Slama N, Laval F, Vaubourgeix J, Crew RM, Gicquel B, Daffé M, Morbidoni HR, Brennan PJ, Quémard A, McNeil MR, Jackson M. A common mechanism of inhibition of the Mycobacterium tuberculosis mycolic acid biosynthetic pathway by isoxyl and thioacetazone. J Biol Chem. 2012;287(46):38434-41.
Layre E, Paepe DC, Larrouy-Maumus G, Vaubourgeix J, Mundayoor S, Lindner B, Puzo G, Gilleron M. Deciphering sulfoglycolipids of Mycobacterium tuberculosis. J Lipid Res. 2011 Jun;52(6):1098-110.
Jang J, Stella A, Boudou F, Levillain F, Darthuy E, Vaubourgeix J, Wang C, Bardou F, Puzo G, Gilleron M, Burlet-Schiltz O, Monsarrat B, Brodin P, Gicquel B, Neyrolles O. Functional characterization of the Mycobacterium tuberculosis serine/threonine kinase PknJ. Microbiology. 2010 Jun;156:1619-31.
Vaubourgeix J, Bardou F, Boissier F, Julien S, Constant P, Ploux O, Daffé M, Quémard A, Mourey L. Sadae, a potent bisubstrate inhibitor of Mycobacterium tuberculosis mycolic acid methyltransferases. J Biol Chem. 2009 Jul;284(29):19321-30.
Peyron P*, Vaubourgeix J*, Poquet Y, Levillain F, Botanch C, Bardou F, Daffé M, Emile JF, Marchou B, Cardona PJ, de Chastellier C, Altare F. Foamy macrophages from tuberculous patients' granulomas constitute a nutrient-rich reservoir for M. tuberculosis persistence. PLoS Pathogens. 2008 Nov;4(11):e1000204.
Reviews and Perspectives
Schrader SM, Botella H, Vaubourgeix J. Re-framing antimicrobial resistance as a continuous spectrum of manifestations. Curr Opin in Microbiol. 2023 Jan 4;72:102259.
Schrader SM*, Vaubourgeix J*, Nathan C. Biology of Antimicrobial Resistance and Approaches to Combat It. Sci Transl Med. 2020 Jun 24;12(549):eaaz6992.
Botella H, Vaubourgeix J. Building Walls: Work That Never Ends. Trends Microbiology. 2019 Jan;27(1):4-7.
Lupoli T*, Vaubourgeix J*, Burns-Huang K*, Gold B. Targeting the proteostasis network for mycobacterial drug discovery. ACS Inf Diseases. DOI: 10.1021/acsinfecdis.7b00231, February 2018.Cliquer ici pour modifier.
Schrader SM, Botella H, Jansen R, Ehrt S, Rhee K, Nathan C#, Vaubourgeix J#. Multiform antimicrobial resistance from a metabolic mutation. Science Advances. 2021 Aug 27;7(35):eabh2037. Lead corresponding author.
Harnagel A, Lopez Quezada L, Park SW, Baranowski C, Kieser K, Jiang X, Roberts J, Vaubourgeix J, Yang A, Nelson B, Fay Allison, Rubin E, Ehrt S, Nathan C, Lupoli TJ. Nonredundant functions of Mycobacterium tuberculosis chaperones promote survival under stress. Mol Microbiol. 2021 Feb;115(2):272-289.
Wang R, Kreutzfeldt K, Botella H, Vaubourgeix J, Schnappinger D, Ehrt S. Persistent Mycobacterium tuberculosis infection in mice requires PerM for successful cell division. Elife. 2019 Nov;8:e49570.
McKenney P, Yan J, Vaubourgeix J, Becattini S, Lampen N, Motzer A, Larson PJ, Dannoui D, Fujisawa S, Xavier JB, Pamer EG. Intestinal bile acids induce a morphotype switch in vancomycin resistant Enterococcus that facilitates intestinal colonization. Cell Host Microbe. 2019 May 8;25(5):695-705.
Ballinger E, Mosior J, Hartman T, Burns-Huang K, Gold B, Morris R, Goullieux L, Blanc I, Vaubourgeix J, Lagrange S, Fraisse L, Sans S, Couturier C, Bacqué E, Rhee K, Scarry SM, Aubé J, Yang G, Ouerfelli O, Schnappinger D, Ioerger TR, Engelhart CA, McConnell JA, McAulay K, Fay A, Roubert C, Sacchettini J, Nathan C. Opposing reactions in coenzyme A metabolism sensitize Mycobacterium tuberculosis to enzyme inhibition. Science. 2019 Feb 1;363(6426).
Botella H, Yang G, Ouerfelli O, Ehrt S, Nathan C, Vaubourgeix J. Distinct spatio-temporal dynamics in peptidoglycan synthesis between M. smegmatis and M. tuberculosis. mBio. 2017 Sep 12;8(5).
Botella H, Vaubourgeix J, Song N, Xu W, Makinoshima H, Lee MH, Glickman MS and Ehrt S. Mycobacterium tuberculosis periplasmic protease MarP activates the peptidoglycan hydrolase RipA during acid stress. EMBO J. 2017 Feb 15;36(4):536-548.
Botella L, Vaubourgeix J, Livny J, Schnappinger D. Depleting Mycobacterium tuberculosis of the transcription termination factor Rho causes pervasive transcription and rapid death. Nature Communications. 2017 March 28; 8:14731.
Goodsmith N, Guo XV, Vandal OH, Vaubourgeix J, Wang R, Botella H, Song S, Bhatt K, Liba A, Salgame P, Schnappinger D, Ehrt S. Disruption of an M. tuberculosis membrane protein causes a magnesium-dependent cell division defect and failure to persist in mice. PLoS Pathogens. 2015 Feb 6;11(2):e1004645.
Vaubourgeix J, Lin G, Dhar N, Chenouard N, Jiang X, Botella H, Lupoli T, Mariani O, Yang G, Ouerfelli O, Unser M, Schnappinger D, McKinney J, Nathan C. Stressed mycobacteria use the chaperone ClpB to sequester irreversibly oxidized proteins asymmetrically within and between cells. Cell Host Microbe. 2015 Feb 11;17(2):178-90.
Lin G, Chidawanyika T, Tsu C, Warrier T, Vaubourgeix J, Blackburn C, Gigstad K, Sintchak M, Dick L, Nathan C. N,C-Capped dipeptides with selectivity for mycobacterial proteosome over human proteasomes: role of S3 and S1 binding pockets. J Am Chem Soc. 2013;135(27):9968-71.
Grzegorzewicz AE, Korduláková J, Jones V, Born SE, Belardinelli JM, Vaquié A, Gundi VA, Madacki J, Slama N, Laval F, Vaubourgeix J, Crew RM, Gicquel B, Daffé M, Morbidoni HR, Brennan PJ, Quémard A, McNeil MR, Jackson M. A common mechanism of inhibition of the Mycobacterium tuberculosis mycolic acid biosynthetic pathway by isoxyl and thioacetazone. J Biol Chem. 2012;287(46):38434-41.
Layre E, Paepe DC, Larrouy-Maumus G, Vaubourgeix J, Mundayoor S, Lindner B, Puzo G, Gilleron M. Deciphering sulfoglycolipids of Mycobacterium tuberculosis. J Lipid Res. 2011 Jun;52(6):1098-110.
Jang J, Stella A, Boudou F, Levillain F, Darthuy E, Vaubourgeix J, Wang C, Bardou F, Puzo G, Gilleron M, Burlet-Schiltz O, Monsarrat B, Brodin P, Gicquel B, Neyrolles O. Functional characterization of the Mycobacterium tuberculosis serine/threonine kinase PknJ. Microbiology. 2010 Jun;156:1619-31.
Vaubourgeix J, Bardou F, Boissier F, Julien S, Constant P, Ploux O, Daffé M, Quémard A, Mourey L. Sadae, a potent bisubstrate inhibitor of Mycobacterium tuberculosis mycolic acid methyltransferases. J Biol Chem. 2009 Jul;284(29):19321-30.
Peyron P*, Vaubourgeix J*, Poquet Y, Levillain F, Botanch C, Bardou F, Daffé M, Emile JF, Marchou B, Cardona PJ, de Chastellier C, Altare F. Foamy macrophages from tuberculous patients' granulomas constitute a nutrient-rich reservoir for M. tuberculosis persistence. PLoS Pathogens. 2008 Nov;4(11):e1000204.
Reviews and Perspectives
Schrader SM, Botella H, Vaubourgeix J. Re-framing antimicrobial resistance as a continuous spectrum of manifestations. Curr Opin in Microbiol. 2023 Jan 4;72:102259.
Schrader SM*, Vaubourgeix J*, Nathan C. Biology of Antimicrobial Resistance and Approaches to Combat It. Sci Transl Med. 2020 Jun 24;12(549):eaaz6992.
Botella H, Vaubourgeix J. Building Walls: Work That Never Ends. Trends Microbiology. 2019 Jan;27(1):4-7.
Lupoli T*, Vaubourgeix J*, Burns-Huang K*, Gold B. Targeting the proteostasis network for mycobacterial drug discovery. ACS Inf Diseases. DOI: 10.1021/acsinfecdis.7b00231, February 2018.Cliquer ici pour modifier.