Mini CV
EDUCATION & DIPLOMAS
PROFESSIONAL EXPERIENCE
-Project manager for studying the stress effect in the regulation of gut proteolytic equilibrium and barrier function
-Project manager for the development of a therapeutical strategy to alleviate intestinal inflammation
-Project manager for studying redox regulation of pathogenicity islands in enteric bacteria
-Project manager for studying the influence of the host microbiota on M. tuberculosis infection and anti-TB response
-Project manager for identifying M. tuberculosis genes involved in trophic interactions with the microbiota
·- Study of microbial nutrient transporters in metabolic adaptation and intracellular survival of Mycobacterium tuberculosis
·- Production and enzymatic characterization of the M. tuberculosis asparaginase AnsA
- 2014-2018: PhD in Microbiology (Paul Sabatier University, Toulouse, FR)
- 2012-2014: Master degree “Structural and functional biochemistry” (Paul Sabatier University, Toulouse, FR)
- 2009-2012: License degree “Biochemistry and molecular biology” (Paul Sabatier University, Toulouse, FR)
PROFESSIONAL EXPERIENCE
- From August 2021: Post-Doctoral Research – Institute of Research in Digestive Health (IRSD), FR, in Dr. Nathalie Vergnolle’s lab
-Project manager for studying the stress effect in the regulation of gut proteolytic equilibrium and barrier function
- 2019-2021: Post-Doctoral Research – University College Dublin (UCD), IE, in Prof. Ulla Knaus’ lab
-Project manager for the development of a therapeutical strategy to alleviate intestinal inflammation
-Project manager for studying redox regulation of pathogenicity islands in enteric bacteria
- 2014-2018: Doctoral Research (PhD) – Institute of Pharmacology and Structural Biology (IPBS), CNRS-University of Toulouse, FR, in Dr. Olivier Neyrolles’ lab
-Project manager for studying the influence of the host microbiota on M. tuberculosis infection and anti-TB response
-Project manager for identifying M. tuberculosis genes involved in trophic interactions with the microbiota
- 2012-2014: Master internships – IPBS, Toulouse, FR, in Dr. Olivier Neyrolles’ lab
·- Study of microbial nutrient transporters in metabolic adaptation and intracellular survival of Mycobacterium tuberculosis
·- Production and enzymatic characterization of the M. tuberculosis asparaginase AnsA
expertise
- Microbiology: Study of E. coli, C. rodentium (BSL1); S. pneumoniae, E. faecalis, Enteropathogenic E. coli (BSL2); and M. tuberculosis (BSL3) bacterial strains
Molecular biology: Gene cloning & mutagenesis in E. coli and M. tuberculosis; RNA/DNA extraction from bacteria and mouse tissues; PCR & RT-qPCR
Biochemistry: Protein production & purification; enzyme activity assays (absorbance, fluorescence, chemiluminescence); western-blot & TLC analyses
Animal experimentation: Mouse model; experimentation in BSL1, BSL2, BSL3 facilities; health scoring system for colitis models (TNBS, DSS); intranasal, intraperitoneal & intrarectal injections; oral gavage; euthanasia; organ dissection (lungs, liver, spleen, GIT) & preparation for histological analysis
Cell biology: Mammalian cell culture (primary cells & cell lines); cytokine measurement (ELISA & LegendplexTM); cell preparation from mouse organs (lungs, liver, spleen, lamina propria) for immune monitoring by flow cytometry
Recherche/projet research/project
Role of stress in the regulation of gut proteolytic equilibrium and barrier function
Irritable Bowel Syndrome (IBS) is a gastrointestinal disorder commonly defined by abdominal pain and disturbances in bowel habits. The pathophysiology of IBS is not completely resolved, but it is well reported that there is a disordered communication between the gut and the brain. In particular, the sensory neurons innervating the colon are hyperexcitable in these patients, leading to visceral hypersensitivity, and exacerbating the sensation of pain.
Our lab is specialized in the study of key mediators of the intestinal mucosa, the gut proteases, and more generally on the role of the proteolytic equilibrium in health and disease (i.e. cellular sources, regulatory mechanisms, and targets involved). The lab has contributed many times to show that the regulation of the proteolytic activity is essential for gut homeostasis, and that an unbalance is associated with several pathological states, including IBS. For instance, a proteolytic activity increased is found in colon of IBS patients, able to signal to enteric neurons via a protease-activated receptor-2-dependent mechanism, inducing visceral hypersensitivity.
Psychological stress is an important factor of the gut-brain axis, contributing in the development of IBS. Preliminary data in our lab suggest that chronic stress exposure alters proteolytic activity. This project aims to explore the molecular mechanisms enabling the stress response pathway (i.e. stress-associated molecules) to impair gut proteolytic equilibrium, and study the consequences on the barrier function and visceral hypersensitivity, characteristic disorder found in IBS patients.
Irritable Bowel Syndrome (IBS) is a gastrointestinal disorder commonly defined by abdominal pain and disturbances in bowel habits. The pathophysiology of IBS is not completely resolved, but it is well reported that there is a disordered communication between the gut and the brain. In particular, the sensory neurons innervating the colon are hyperexcitable in these patients, leading to visceral hypersensitivity, and exacerbating the sensation of pain.
Our lab is specialized in the study of key mediators of the intestinal mucosa, the gut proteases, and more generally on the role of the proteolytic equilibrium in health and disease (i.e. cellular sources, regulatory mechanisms, and targets involved). The lab has contributed many times to show that the regulation of the proteolytic activity is essential for gut homeostasis, and that an unbalance is associated with several pathological states, including IBS. For instance, a proteolytic activity increased is found in colon of IBS patients, able to signal to enteric neurons via a protease-activated receptor-2-dependent mechanism, inducing visceral hypersensitivity.
Psychological stress is an important factor of the gut-brain axis, contributing in the development of IBS. Preliminary data in our lab suggest that chronic stress exposure alters proteolytic activity. This project aims to explore the molecular mechanisms enabling the stress response pathway (i.e. stress-associated molecules) to impair gut proteolytic equilibrium, and study the consequences on the barrier function and visceral hypersensitivity, characteristic disorder found in IBS patients.
Production scientifique scientific production
Dumas et al. Tn-Seq screening reveals the importance of Mycobacterium tuberculosis solute transport systems in mycobacterial interaction with the host microbiota. Manuscript in preparation
A Pulmonary Lactobacillus murinus Strain Induces Th17 and RORγt+ Regulatory T Cells and Reduces Lung Inflammation in Tuberculosis.
Bernard-Raichon L, Colom A, Monard SC, Namouchi A, Cescato M, Garnier H, Leon-Icaza SA, Métais A, Dumas A, Corral D, Ghebrendrias N, Guilloton P, Vérollet C, Hudrisier D, Remot A, Langella P, Thomas M, Cougoule C, Neyrolles O, Lugo-Villarino G.J Immunol. 2021 Oct 1;207(7):1857-1870. doi: 10.4049/jimmunol.2001044. Epub 2021 Sep 3.PMID: 34479945
Raising the 'Good' Oxidants for Immune Protection.
Dumas A, Knaus UG.Front Immunol. 2021 Jun 4;12:698042. doi: 10.3389/fimmu.2021.698042. eCollection 2021.PMID: 34149739 Free PMC article. Review.
The Host Microbiota Contributes to Early Protection Against Lung Colonization by Mycobacterium tuberculosis.
Dumas A, Corral D, Colom A, Levillain F, Peixoto A, Hudrisier D, Poquet Y, Neyrolles O.Front Immunol. 2018 Nov 14;9:2656. doi: 10.3389/fimmu.2018.02656. eCollection 2018.PMID: 30487801 Free PMC article.
The role of the lung microbiota and the gut-lung axis in respiratory infectious diseases.
Dumas A, Bernard L, Poquet Y, Lugo-Villarino G, Neyrolles O.Cell Microbiol. 2018 Dec;20(12):e12966. doi: 10.1111/cmi.12966. Epub 2018 Oct 30.PMID: 30329198 Review.
Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection.
Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, Guerquin-Kern JL, Schnappinger D, Sório de Carvalho LP, Poquet Y, Neyrolles O.PLoS Pathog. 2014 Feb 20;10(2):e1003928. doi: 10.1371/journal.ppat.1003928. eCollection 2014 Feb.PMID: 24586151 Free PMC article.
A Pulmonary Lactobacillus murinus Strain Induces Th17 and RORγt+ Regulatory T Cells and Reduces Lung Inflammation in Tuberculosis.
Bernard-Raichon L, Colom A, Monard SC, Namouchi A, Cescato M, Garnier H, Leon-Icaza SA, Métais A, Dumas A, Corral D, Ghebrendrias N, Guilloton P, Vérollet C, Hudrisier D, Remot A, Langella P, Thomas M, Cougoule C, Neyrolles O, Lugo-Villarino G.J Immunol. 2021 Oct 1;207(7):1857-1870. doi: 10.4049/jimmunol.2001044. Epub 2021 Sep 3.PMID: 34479945
Raising the 'Good' Oxidants for Immune Protection.
Dumas A, Knaus UG.Front Immunol. 2021 Jun 4;12:698042. doi: 10.3389/fimmu.2021.698042. eCollection 2021.PMID: 34149739 Free PMC article. Review.
The Host Microbiota Contributes to Early Protection Against Lung Colonization by Mycobacterium tuberculosis.
Dumas A, Corral D, Colom A, Levillain F, Peixoto A, Hudrisier D, Poquet Y, Neyrolles O.Front Immunol. 2018 Nov 14;9:2656. doi: 10.3389/fimmu.2018.02656. eCollection 2018.PMID: 30487801 Free PMC article.
The role of the lung microbiota and the gut-lung axis in respiratory infectious diseases.
Dumas A, Bernard L, Poquet Y, Lugo-Villarino G, Neyrolles O.Cell Microbiol. 2018 Dec;20(12):e12966. doi: 10.1111/cmi.12966. Epub 2018 Oct 30.PMID: 30329198 Review.
Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection.
Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, Guerquin-Kern JL, Schnappinger D, Sório de Carvalho LP, Poquet Y, Neyrolles O.PLoS Pathog. 2014 Feb 20;10(2):e1003928. doi: 10.1371/journal.ppat.1003928. eCollection 2014 Feb.PMID: 24586151 Free PMC article.