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​


CHERCHEUR

INSERM
​CRHC

Permanent researcher


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ORCID iD QR Code

CONTACTS

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​00 33 (0)5 62 74 45 73
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EQUIPE 4 : METABOLISME DU FER: DE LA REGULATION A LA THERAPIE 
TEAM 4 :  IRON METABOLISM:FROM REGULATION TO THERAPY ​

Mini CV

EDUCATION
  • 2006: National Diploma of Habilitation to Direct Research (HDR) in Life science and Health , University  Paris VII
  • 1991/96 : Ph.D. in Molecular and Cellular Biology, ULP, Strasbourg, France
  • 1989/90 : Master's degree in Molecular and Cellular Biology, ULP, Strasbourg, France
  • 1986/89 : Bachelor of Science in Cellular Biology and Physiology, ULP, Strasbourg, France
RESEARCH  EXPERIENCE
  • 2021- Présent:  Senior Scientist (CRHC) INSERM, U1220, IRSD,  Toulouse, France
  • 2016-2021: Senior Scientist (CRCN) INSERM, U1220, IRSD,  Toulouse, France
  • 2011-2016  : Senior Scientist (CR1) INSERM, U1043, CPTP,  Toulouse, France
  • 2007-2010 :  Senior Scientist (CR1) INSERM, UPR2301, CNRS,  ICSN, Gif-Sur-Yvette, France
  • 2006-2007:    Senior Scientist (CR1) INSERM, U773, Faculté Xavier Bichat,  Paris, France
  • 2005-2006:     Senior Scientist (CR2) INSERM, U656, Faculté Xavier Bichat,  Paris, France
  • 2002-2005:    Senior Scientist (CR2) INSERM, U409, Faculté Xavier Bichat,  Paris, France
  • 1997/2002:     Post-doctoral research in the laboratory of Professor Philippe Gros, Department of Biochemistry, University McGill, Quebec, Canada.

 RESEARCH  SUPPORT EXPERIENCE  

Scientific integrity and good research practices

​​​Involved during several years as a whistleblower in a major misconduct research case in my scientific domain of expertise, I followed an INSERM formation and I used my experience and knowledge to develop this great and important mission in research support in the name of INSERM.    (Conceptor-Designer of webpage dedicated to research integrity, create awareness  and educate thought documents, courses, and seminars ,  young and mature researchers to all aspect of misconduct research. )​
activity in Research Integrity

OTHER RESEARCH  SUPPORT EXPERIENCE  
​
Since the creation of the IRSD in 2016, I was identified as the correspondent of several actions for the credit of IRSD members as well as local and national research community : 
  •  Scientific communication correspondent : Conceptor -designer  and administrator (Webmaster) of the Inserm 1220 website, computer graphics work with creation of images, diagrams and logo for the teams.
https://www.irsd.fr/
The website is a important communication tool  with : Progress report &  seminars announcement, calendars/agenda, data exchange (publication, thematic), scientific and technological watch and collaborative work (contact). The website presents the institute, its values, its objectives (global and by team), its services (administration, logistics), its services (platforms), its productions (discoveries, publication) and makes it possible to increase the visibility of the institute. 
  • Scientific  animation correspondent (seminar and progress report organization, scientific retreat, other events...)
https://www.irsd.fr/animation.html
I led a committee at IRSD to organise both scientific and "team "building" activities in the institute in order to create a convivial  atmosphere of scientific excellence .  
  • ​Sustainable development correspondent 
https://ddcptpirsd.weebly.com/
I recently led a committee dedicated to the reflection and implementation of measures (energy saving, transport, recycling) to limit the impact on the environment of our activities.
  • Creation and animation of  The  Macrophage club in Occitania / Organization of symposia
My scientific curiosity for macrophages allowed me to develop a thematic and methodological expertise targeted for these cells and led me to found with other colleagues the Club du Macrophage de Toulouse and  then thereafter a monocyte-macrophage network extended to the Occitanie cultural region (https://macrophagecluboccitanie.weebly.com/) grouping the cities of Toulouse, Montpellier, Bordeaux, Nice and Marseille. I organize since 2016 the annual symposium SoMM (Symposium of the Occitanie Network on Monocytes-macrophages) of this community.

Scientific expertise

Animal and Cellular models:
  • Mouse models of iron disorders –(iron overload and iron deficiency)
  • Murine Bone marrow derived macrophages
  • LCCM (L929 conditioned culture medium to produce M-CSF1) for BMDM culture
  • Murine macrophages cell lines : J774a1; Raw264.7

Techniques and Methods:
  • *Rabbit polyclonal antibodies production and purification by affinity (see the Note) 
  •   Protein expression and localization
  • Crude membrane versus cytosolic extracts (ultracentrifugation)
  • lipid rafts analysis
  • Western blot
  • In cell western blotting (Odyssey)
  • Immunofluorescence on fixed cells
  • Immunohistochemistry and immunohistofluorescence on paraffin  tissues section
  • In vitro erythrophagocytosis assay (PMID: 16095591)
  • In vivo and in vitro salmonella infection
  • *NOTE : Major expertice : Purification, characterization and use of polyclonal antibodies against iron transporters.​
The affinity purification of the various custom antibodies obtained in rabbits requires the production and purification of recombinant proteins in E. coli used for the specific retention and elution of the antibodies present in rabbit serum (affinity column). As part of this program, I supervised several students and members of my team to  carried out the purification of several antibodies directed against different murine and human iron transporters.
These tools and their use, allowed me to acquire an international recognized expertise in the detection of iron transporters ex vivo in cell culture and in vivo in mice (expression and localization analysis; western blot, immunofluorescence, electron microscopy ...).
Many research groups encounter difficulties in finding specific antibodies in the trade and having neither the means nor the expertise to develop them. So they contact me to request certain antibodies needed  for their studies as well as my expertise in acquisition (protocols) and analysis of the detection of iron transporters (collaboration in the form of a Material Transfer Agreement MTA).

RESEARCH topics

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MACROPHAGE IRON METABOLISM AND PATHOLOGIES. 
​My research focuses on  mammalian iron homeostasis and in particular in the key processes of heme iron storage and recycling from aged or damaged red blood cells by macrophages. For this purpose I developed a physiological model that mimics the process of erythrophagocytosis including red blood cells ageing, recognition and engulfment by macrophages. Using this model, we studied the different steps of heme iron recycling by macrophages①. We clarified important regulation at both mRNA and protein levels of Ferroportin (FPN), the only mammalian iron exporter identified to date. We also showed that Hepcidin (HAMP), a small peptide considered as the major hormonal regulator of iron homeostasis, induces a rapid internalization and degradation of the macrophage iron exporter present at the cell surface of macrophages. To get insight into the molecular mechanisms involved in HAMP mediated downregulation of FPN protein, we developed cellular and proteomic approaches to better define the environment of FPN in macrophage cellular membranes②. We also studied the relation between macrophage iron recycling, infection with intracellular pathogens (such as salmonella) and anemia of inflammation③.
 I am also interested in the implication of macrophagic iron in:
- Atherogenesis (Marques et al, BBA2016). Our results indicate a decrease in the export of macrophagic iron via FPN under atherogenic conditions (inflammation and oxLDL). This decrease would lead to an increase in intracellular iron in macrophages and could thus contribute to the accumulation of iron in the atheroma plaque and its destabilization.
-Sensitivity to salmonella infection (Willemetz et al, Front Immunol, 2017) via analysis of the FPN-HAMP regulatory axis. Our ex vivo (macrophage culture) and in vivo (mouse iron pathology models) results clearly demonstrate a downregulation of FPN during salmonella infection. Our work is in opposition to the literature and raise new questions on inflammatory infectious anemias and their therapies.

links

Le club Macrophage d'Occitanie
​ResearhGate Canonne-Hergaux 
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LinkedIn Canonne-Hergaux

SCIENTIFIC PRODUCTION

​​Publications issues du Web of Sciences – Core Collection
ResearcherID:  
AAC-2073-2020  
URL:   
https://publons.com/researcher/3347963/francois-canonne-hergaux/
ORCID:            0000-0002-0621-2157
​PubMed Canonne-Hergaux 
​
FullText in ResearchGate 

pictures

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phagocytes aggregation in iron overload liver

​Immunohistofluorescence in liver section. 
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Iron in the liver

Iron  is visualized in histological section of the liver after a Perls Prussian Blue staining

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INSERM IRSD U1220 , CHU PURPAN PLACE DU DOCTEUR BAYLAC, 
​CS 60039 31024, TOULOUSE CEDEX 3 
 00 33 (0)5 62 74 45 03   

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Webmaster: François Canonne-Hergaux