MINI CV
Depuis 2020 CRCN, équipe 6 - Audrey Ferrand et Frédérick Barreau, INSERM 1220.
2011-2020 CRCN, équipe Vassilia Theodorou, INRAE Toxalim, Toulouse. « Conséquences à long terme d’effets adverses dans la petite enfance ».
2007-2010 Post-doc, équipe INSERM Nadine Cerf Bensussan, IRNEM Necker, Paris. « Absorption intestinale du gluten chez les malades cœliaques actifs ».
2005-2007 Post-doc Karolinska institute, Stockholm, Suède/Institute for medical Microbioloy, Freiburg, Allemagne. Mathias Hornef. « Expression développementale des peptides antimicrobiens entériques. »
2004 Thèse de sciences en physiopathologie. Equipe INSERM, Martine Heyman IRNEM, Necker, Paris.« Effets bénéfiques des bactéries probiotiques. »
Since 2020 CRCN, team 6 - Audrey Ferrand et Frédérick Barreau, INSERM 1220.
2011-2020 CRCN, team Vassilia Theodorou, INRAE Toxalim, Toulouse. « Long time consequences of early life adverse events ».
2007-2010 Post-doc, équipe INSERM Nadine Cerf Bensussan, IRNEM Necker, Paris. « Gluten intestinal absorption in active coeliac patients ».
2005-2007 Post-doc Karolinska institute, Stockholm, Suède/Institute for medical Microbioloy, Freiburg, Allemagne. Mathias Hornef. « Developmental expression of enteric antimicrobial peptides »
2004 PhD physiopathology. Team INSERM, Martine Heyman IRNEM, Necker, Paris. « Beneficial effects of probiotic bacteria »
2011-2020 CRCN, équipe Vassilia Theodorou, INRAE Toxalim, Toulouse. « Conséquences à long terme d’effets adverses dans la petite enfance ».
2007-2010 Post-doc, équipe INSERM Nadine Cerf Bensussan, IRNEM Necker, Paris. « Absorption intestinale du gluten chez les malades cœliaques actifs ».
2005-2007 Post-doc Karolinska institute, Stockholm, Suède/Institute for medical Microbioloy, Freiburg, Allemagne. Mathias Hornef. « Expression développementale des peptides antimicrobiens entériques. »
2004 Thèse de sciences en physiopathologie. Equipe INSERM, Martine Heyman IRNEM, Necker, Paris.« Effets bénéfiques des bactéries probiotiques. »
Since 2020 CRCN, team 6 - Audrey Ferrand et Frédérick Barreau, INSERM 1220.
2011-2020 CRCN, team Vassilia Theodorou, INRAE Toxalim, Toulouse. « Long time consequences of early life adverse events ».
2007-2010 Post-doc, équipe INSERM Nadine Cerf Bensussan, IRNEM Necker, Paris. « Gluten intestinal absorption in active coeliac patients ».
2005-2007 Post-doc Karolinska institute, Stockholm, Suède/Institute for medical Microbioloy, Freiburg, Allemagne. Mathias Hornef. « Developmental expression of enteric antimicrobial peptides »
2004 PhD physiopathology. Team INSERM, Martine Heyman IRNEM, Necker, Paris. « Beneficial effects of probiotic bacteria »
EXPERTISE
- Intestinal barrier functions : intestinal permeability Ussing and in vivo (para, trans, receptor mediated), antimicrobial peptides, cellular and humoral immunity.
- Intestinal physiology and pathophysiology
- Food additives
- Enteric infection
- Social stress
- Developmental Origin of health and diseases (DOHaD)
Projets de recherche / research projects :
Rôle des effets adverses dans la petite enfance sur le développement des maladies chroniques non transmissibles.
Le nombre de personnes atteintes de maladies chroniques non transmissibles (NCDs) ne cesse de croitre dans le monde. Les origines développementales de la santé et des maladies (DOHaD) est un concept qui pourrait contribuer au développement des NCDs. En effet, les périodes pré et post natales représentent des fenêtres critiques de maturation pour l’organisme. Des études épidémiologiques et en expérimentation animale suggèrent que la survenue de facteurs exogènes au cours de la période périnatale pourrait influencer la survenue des NCDs chez l’adulte. La période périnatale est critique pour la mise en place de la physiologie intestinale et la tolérance orale vis-à-vis des antigènes luminaux intestinaux (microbiote et antigènes alimentaires). L’occurrence d’évènements adverses périnataux a des effets délétères à long terme sur les mécanismes immuno-régulateurs qui interrogent leur rôle dans la susceptibilité aux intolérances alimentaires (antigènes alimentaires) et Maladie Inflammatoires Chroniques de l’Intestin – MICI (microbiote). Nous avons pour but d’analyser les conséquences des évènements adverses périnataux comme, le stress social, les infections entériques et l’exposition aux contaminants alimentaires, sur l’homéostasie immunitaire et la barrière intestinale qui pourraient contribuer au développement d’intolérances alimentaires ou de MICI.
Role of Early Life Adverse Events in occurrence of Non Communicable Diseases (NCDs).
Non Communicable Diseases (NCDs) are increasing worldwide. The developmental origin of health and Diseases (DOHaD) is a concept that might contribute to development of NCDs as pre and postnatal periods represent a critical time window of maturation for the organism. Complementary data coming from epidemiological studies and experimental animal studies suggest that exogenous factors during perinatal life influence the life-long susceptibility to NCDs in adulthood. Perinatal period represents a critical time window for establishment of intestinal physiology and oral tolerance to intestinal luminal content (microbiota and food antigens). Early Life Adverse Events (ELAE) have long-lasting effects and disturb the establishment of immunoregulatory mechanisms that question their role in susceptibility to adverse reactions toward luminal content : food antigens (adverse food reactions) or microbiota (Inflammatory Bowel Diseases – IBD). We aim at analyzing the influence of ELAE such as social stressful events, oral contaminants or infection, on immune homeostasis and intestinal barrier functions contributing to food adverse reactions or IBD.
Le nombre de personnes atteintes de maladies chroniques non transmissibles (NCDs) ne cesse de croitre dans le monde. Les origines développementales de la santé et des maladies (DOHaD) est un concept qui pourrait contribuer au développement des NCDs. En effet, les périodes pré et post natales représentent des fenêtres critiques de maturation pour l’organisme. Des études épidémiologiques et en expérimentation animale suggèrent que la survenue de facteurs exogènes au cours de la période périnatale pourrait influencer la survenue des NCDs chez l’adulte. La période périnatale est critique pour la mise en place de la physiologie intestinale et la tolérance orale vis-à-vis des antigènes luminaux intestinaux (microbiote et antigènes alimentaires). L’occurrence d’évènements adverses périnataux a des effets délétères à long terme sur les mécanismes immuno-régulateurs qui interrogent leur rôle dans la susceptibilité aux intolérances alimentaires (antigènes alimentaires) et Maladie Inflammatoires Chroniques de l’Intestin – MICI (microbiote). Nous avons pour but d’analyser les conséquences des évènements adverses périnataux comme, le stress social, les infections entériques et l’exposition aux contaminants alimentaires, sur l’homéostasie immunitaire et la barrière intestinale qui pourraient contribuer au développement d’intolérances alimentaires ou de MICI.
Role of Early Life Adverse Events in occurrence of Non Communicable Diseases (NCDs).
Non Communicable Diseases (NCDs) are increasing worldwide. The developmental origin of health and Diseases (DOHaD) is a concept that might contribute to development of NCDs as pre and postnatal periods represent a critical time window of maturation for the organism. Complementary data coming from epidemiological studies and experimental animal studies suggest that exogenous factors during perinatal life influence the life-long susceptibility to NCDs in adulthood. Perinatal period represents a critical time window for establishment of intestinal physiology and oral tolerance to intestinal luminal content (microbiota and food antigens). Early Life Adverse Events (ELAE) have long-lasting effects and disturb the establishment of immunoregulatory mechanisms that question their role in susceptibility to adverse reactions toward luminal content : food antigens (adverse food reactions) or microbiota (Inflammatory Bowel Diseases – IBD). We aim at analyzing the influence of ELAE such as social stressful events, oral contaminants or infection, on immune homeostasis and intestinal barrier functions contributing to food adverse reactions or IBD.
Modèles animaux et cellulaires / ANIMAL AND CELLULAR MODELS
- Early life models of social stress, infections and exposure to contaminants
- Isolation and primary culture of immune cells from: lamina propria, mesenteric lymph node, adipose tissues.
- Polarized and differenciated monolayer of HT29 and Caco-2 cell lines
Techniques et méthodes / TECHNICS AND METHODS
Chambre de Ussing. Mesure de flux paracellulaire, transcellulaire (intact et total), résistance électrique, différence de potentiel et courant de court circuit sur tissus intestinaux de différentes espèces (adultes et nouveaux nés).
Transport de bactéries et réponse au glucose.
Ussing chambers: measurement of paracellular and transcellular (intact and total) fluxes, electrical resistance, potential difference and short circuit current on intestinal fragments from various species (adults and neonates).
Transport of bacteria and intestinal response to glucose challenge.
Transport de bactéries et réponse au glucose.
Ussing chambers: measurement of paracellular and transcellular (intact and total) fluxes, electrical resistance, potential difference and short circuit current on intestinal fragments from various species (adults and neonates).
Transport of bacteria and intestinal response to glucose challenge.
production scientifique / scientific production
Original manuscripts :
Reviews and book chapters:
Congress summary :
- Ilchmann-Diounou H, Buléon M, Bacquié V, Théodorou V, Denis C, Ménard S. Revisiting definition and assessment of intestinal trans-epithelial passage. CMLS. Cell Mol Life Sci. 2021 Dec;78(24):8157-8164. doi: 10.1007/s00018-021-04000-8. Epub 2021 Nov 3.
- Payros D, Alassane-Kpembi I, Laffitte J, Lencina C, Neves M, Bracarense AP, Pinton P, Ménard S, Oswald I. Dietary exposure to the food contaminant deoxynivalenol triggers colonic breakdown by activating the mitochondrial and the death receptor pathways. Molecular Nutrition & Food Research. 2021 Dec;65(23):e2100191. doi: 10.1002/mnfr.202100191. Epub 2021 Oct 20.
- Malaisé Y, Lencina C, Cartier C, Olier M, Ménard S and Guzylack-Piriou L. Dermal impregnation of mothers with Bisphenol A, S or F impairs immune responses and gut barrier functions in a dose and sex-specific manner in offspring mice. Scientific Reports Sci Rep. 2021 Jan 18;11(1):1650. doi: 10.1038/s41598-021-81231-6.
- Guesdon W, Pezier T, Ménard S, Nicolosi A, Le Vern Y, Silvestre A, Diana J, Laurent F, Lacroix-Lamandé S. Cryptosporidium parvum subverts antimicrobial activity of CRAMP by reducing its expression in neonatal mice. Microorganisms. 2020 Oct 23;8(11):1635. doi: 10.3390/microorganisms8111635.
- Malaisé Y, Lencina C, Placide F, Bacquié V, Cartier C, Olier M, Buettner M, Wallbrecht M, Ménard S and Guzylack-Piriou L. Oral exposure to bisphenols induced food intolerance and colitis in vivo by modulating immune response in adult mice. Food and Chemical Toxicology. 2020 Dec;146:111773. doi: 10.1016/j.fct.2020.111773. Epub 2020 Oct 1.
- Payros D, Ménard S, Laffitte J, Neves M, Tremblay-Franco M, Luo S, Fouche E, Sninia SP, Theodorou V, Pinton P, Oswald OP. The food contaminant, deoxynivalenol, modulates 1 the Thelper/Treg balance and increases inflammatory bowel diseases. Arch Toxicol. 2020 Sep;94(9):3173-3184. doi: 10.1007/s00204-020-02817
- Malaisé Y, Lencina C, Cartier C, Olier M, Ménard S and Guzylack-Piriou L. Perinatal oral exposure to low doses of bisphenol A, S or F impairs immune functions at intestinal and systemic levels in female offspring mice. Environ Health. 2020 Aug 31;19(1):93. doi: 10.1186/s12940-020-00614-w.
- Lukowicz C, Ellero-Simatos S, Régnier M, Oliviero F, Lasserre F, Polizzi A, Montagner A, Smati S, Boudou F, Lenfant F, Guzylack-Pirou L, Menard S, Barretto S, Fougerat A, Lippi Y, Naylies C, Bertrand-Michel J, Ait-Belgnaoui A, Theodorou V, Marchi N, Gourdy P, Gamet-Payrastre L, Loiseau N, Guillou H, and Mselli-Lakhal L. Dimorphic metabolic and endocrine disorders in mice lacking the constitutive androstane receptor. Sci Rep. 2019 Dec 27;9(1):20169. doi: 10.1038/s41598-019-56570-0
- Ilchmann-Diounou H, Olier M, Lencina C, Riba A, Barretto S, Nankap M, Sommer C, Guillou H, Ellero-Simatos S, Guzylack-Piriou L, Théodorou V, Ménard S. Early life stress induces type 2 diabetes-like features in ageing mice. Brain Behav Immun. 2019 Aug;80:452-463. doi: 10.1016/j.bbi.2019.04.025
- Régnier M, Polizzi A, Lukowicz C, Smati S, Lasserre F, Lippi Y, Naylies C, Laffitte J, Bétoulières C, Montagner A, Ducheix S, Gourbeyre P, Ellero-Simatos S, Menard S, Bertrand-Michel J, Al Saati T, Lobaccaro JM, Burger HM, Gelderblom WC, Guillou H, Oswald IP, Loiseau N. The protective role of liver X receptor (LXR) during fumonisin B1-induced hepatotoxicity. Arch Toxicol. 2018 Nov 17. doi: 10.1007/s00204-018-2345-2
- Riba A, Olier M, Lacroix-Lamandé S, Lencina C, Bacquié V, Harkat C, Van Langendonck N, Gillet M, Cartier C, Baron M, Sommer C, Mallet V, Zill M, Robert H, Laurent F, Ellero-Simatos S, Théodorou V, Ménard S. Early life stress in mice is a suitable model for Irritable Bowel Syndrome but does not predispose to colitis nor increase susceptibility to enteric infections. Brain Behav Immun. 2018 Oct;73:403-415
- Malaisé Y, Menard S, Cartier C, Gaultier E, Lasserre F, Lencina C, Harkat C, Geoffre N, Lakhal L, Castan I, Olier M, Houdeau E, Guzylack-Piriou L. Gut dysbiosis and impairment of immune system homeostasis in perinatally-exposed mice to Bisphenol A precede obese phenotype development. Sci Rep. 2018 Nov 3;7(1):14472.
- Riba A, Olier M, Lacroix-Lamandé S, Lencina C, Bacquié V, Harkat C, Gillet M, Baron M, Sommer C, Mallet V, Salvador-Cartier C, Laurent F, Théodorou V, Ménard S. Paneth Cell Defects Induce Microbiota Dysbiosis in Mice and Promote Visceral Hypersensitivity. Gastroenterology. 2017 Dec;153(6):1594-1606.e2.
- Malaisé Y, Ménard S, Cartier C, Lencina C, Sommer C, Gaultier E, Houdeau E, Guzylack-Piriou L. Consequences of bisphenol a perinatal exposure on immune responses and gut barrier function in mice. Arch Toxicol. 2017 Jul 21.
- Secher T, Payros D, Brehin C, Boury M, Watrin C, Gillet M, Bernard-Cadenat I, Menard S, Theodorou V, Saoudi A, Olier M, Oswald E. Oral tolerance failure upon neonatal gut colonization with Escherichia coli producing the genotoxin colibactin. Infection and Immunity. 2015 Jun; 83(6)
- Ménard S, Guzylack-Piriou L, Lencina C, Leveque M, Naturel M, Sekkal S, Harkat C, Gaultier E, Olier M, Garcia-Villar R, Theodorou V, Houdeau E. Perinatal exposure to a low dose of bisphenol A impaired systemic cellular immune response and predisposes young rats to intestinal parasitic infection. PLoS One. 2014 Nov 21;9(11)
- Menard S, Guzylack-Piriou L, Leveque M, Braniste V, Lencina C, Naturel M, Moussa L, Sekkal S, Harkat C, Gaultier E, Theodorou V, Houdeau E. Food intolerance at adulthood after perinatal exposure to the endocrine disruptor bisphenol A. FASEB J. 2014 Nov;28(11)
- Payros D, Secher T, Boury M, Brehin C, Ménard S, Salvador-Cartier C, Ceevas-Ramos G, Watrin C, Marcq I, Nougayrède JP, Dubois D, Bedu A, Garnier F, Clermont O, Denamur E, Plaisancié P, Theodorou V, Fioramonti J, Olier M, Oswald E. Maternally acquired genotoxic Escherichia coli alters offspring's intestinal homeostasis. Gut Microbes. 2014 Jun 27;5(3).
- Stockinger S, Albers T, Duerr CU, Ménard S, Pütsep K, Andersson M, Hornef MW. Interleukin-13-Mediated Paneth Cell Degranulation and Antimicrobial Peptide Release. J Innate Immun. 2014; 6(4):530-41
- N Khen-Dunlop, S Sarnacki, A Victor, C Grosos, S Ménard, R Soret, N Goudin, M Pousset, F Sauvat, Y Révillon, N Cerf-Bensussan, M Neunlist. Prenatal intestinal obstruction affects the myenteric plexus and causes functional bowel impairment in fetal rat experimental model of intestinal atresia. PLoS One 2013 May 8;8(5).
- Verdier J, Deroche L, Allez M, Loy C, Biet F, Bodier CC, Bay S, Ganneau C, Matysiak-Budnik T, Reyrat JM, Heyman M, Cerf-Bensussan N, Ruemmele FM, Ménard S. Specific IgG Response against Mycobacterium avium paratuberculosis in Children and Adults with Crohn's Disease. PLoS One. 2013 May 2;8(5)
- L Moussa, V Bézirard, C Salvador-Cartier, V Bacquié, C Lencina, M Lévêque, V Braniste, S Ménard, V Théodorou#, E Houdeau#. A low dose of fermented soy germ alleviates gut barrier injury, hyperalgesia and faecal protease activity in a rat model of inflammatory bowel disease. PLOSOne 2012;7(11).
- C Lebreton#, S Ménard#, J Abed, I Cruz-Moura, R Coppo, C Dugave, RC Monteiro, MC Traore, C Cellier, G Malamut, N Cerf-Bensussan, M Heyman. Interactions among Secretory Immunogloblulin A, CD71, and Transglutaminase-2 Affect Permeability of Intestinal Epithelial Cells to Gliadin Peptides. Gastroenterology 2012 Jun 28.
- S Ménard, C Lebreton, M Schumann, T Matysiak-Budnik, C Dugave, Y Bouhnik, G Malamut, C Cellier, M Allez, P Crenn, JD Schulzke, N Cerf-Bensussan and M Heyman. Paracellular versus transcellular intestinal permeability to gliadin peptides in active celiac disease. Am J Pathol 2012 Fev; 180(2):608-15
- S Ménard, V Förster, M Lotz, D Gütle, C Dürr, R L Gallo, B Henriques-Normark, M Andersson, E Glocker, M W. Hornef. Developmental switch of intestinal antimicrobial peptide expression. J Exp Med. 2008 Jan 21;205(1):183-93
- T Matysiak-Budnik, I Cruz Moura, M Arcos-Fajardo, C Lebreton, S Ménard, C Candalh, K Ben-Khalifa, C Dugave, H Tamouza, G van Niel, Y Bouhnik, D Lamarque, S Chaussade, G Malamut, C Cellier, N Cerf-Bensussan, R C. Monteiro and M Heyman. Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease J Exp Med. 2008 Jan 21;205(1):143-54
- Lotz M#, König T#, Ménard S, Gütle D, Bogdan C, Hornef MW. Cytokine-mediated modulation of lipopolysaccharide induced activation of intestinal epithelial cells. Immunology, 2007 Nov; 122, 306–315
- Laharie D, Menard S, Asencio C, Vidal-Martinez T, Rullier A, Zerbib F, Candalh C, Megraud F, Heyman M, Matysiak-Budnik T. Effect of rebamipide on the colonic barrier in interleukin-10-deficient mice. Dig Dis Sci. 2007 Jan;52(1):84-92.
- S. Ménard, C. Candalh, M Ben Ahmed, S. Rakotobe,V. Gaboriau-Routhiau, N. Cerf-Bensussan, M. Heyman. Stimulation of immunity without alteration of oral tolerance in mice fed with heat-treated fermented infant formula. J Pediatr Gastroenterol Nutr. 2006 Oct;43(4):451-8
- Lotz M, Gutle D, Walther S, Ménard S, Bogdan C, Hornef MW. Postnatal acquisition of endotoxin tolerance in intestinal epithelial cells. J Exp Med. 2006 Apr 17;203(4):973-84.
- Menard S, Laharie D, Asensio C, Vidal-Martinez T, Candalh C, Rullier A, Zerbib F, Megraud F, Matysiak-Budnik T, Heyman M. Bifidobacterium breve and Streptococcus thermophilus secretion products enhance T helper 1 immune response and intestinal barrier in mice. Exp Biol Med. 2005 Nov;230(10):749-56.
- JC Bambou, A Giraud, S Ménard, B Begue, S Rakotobe, M Heyman, F Taddei, N Cerf-Bensussan and Gaboriau-Routhiau V. In vitro and ex vivo activation of the TLR5 signaling pathway in intestinal epithelial cells by a commensal Escherichia coli strain. J Biol Chem. 2004 Oct 8; 279(41):42984-92.
- S Ménard, C Candalh, JC Bambou, K Terpend, N Cerf-Bensussan, M Heyman. Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport. Gut 2004;53:821-828.
Reviews and book chapters:
- Barreau F, Tisseyre C, Ménard S, Ferrand A, Carriere M. Titanium dioxide particles from the diet: involvement in the genesis of inflammatory bowel diseases and colorectal cancer. Particle and Fibre Toxicology
- Guzylack-Piriou L, Ménard S. Early Life Exposure to Food Contaminants and Social Stress as Risk Factor for Metabolic Disorders Occurrence?-An Overview. Biomolecules. 2021 May 3;11(5):687. doi: 10.3390/biom11050687.
- Ilchmann-Diounou H and Menard S. Psychological Stress, Intestinal Barrier Dysfunctions and Autoimmune Disorders: an overview. Front. Immunol. 11:1823. Doi: 10.3389/fimmu.2020.01823
- Maresca M, Pinton P, Ajandouz EH, Menard S, Ferrier L, Oswald IP. Overview and Comparison of Intestinal Organotypic Models, Intestinal Cells, and Intestinal Explants Used for Toxicity Studies. Curr Top Microbiol Immunol. 2018 Sep 28. doi: 10.1007/82_2018_142.
- Ménard S, Cerf Bensussan N, Heyman M. Multiple facets of intestinal permeability and epithelial handling of dietary antigens. Mucosal Immunol. 2010 May;3(3):247-59
- Ménard S.#, Lotz M.#, and Hornef MW. Innate immune recognition on the intestinal mucosa. International journal of medical microbiology. 2007 Sep;297(5):379-92
- M. Heyman, K. Terpend. S. Ménard. Effects of specific lactic acid bacteria on the intestinal permeability to macromolecules and the inflammatory condition. Acta Paediatrica Suppl. 2005 Oct;94(449):34-6.
- S. Ménard, M. Heyman. Modulation of epithelial function and local immune system by probiotics: mechanisms involved. Chapter in a book entitled “Probiotics in Food Safety and Human Health” Editor: I. GOKTEPE, Marcel Dekker Inc N.Y. 2005.
- M. Heyman, S. Ménard. Probiotic microorganisms: how they affect intestinal pathophysiology. Cell Mol Life Sci. 2002 Jul;59(7):1151-65.
Congress summary :
- M. Heyman, S. Ménard. Pathways of Gliadin Transport in Celiac Disease. Molecular Structure and Function of the Tight Junction: Ann. N.Y. Acad. Sci. 1165: 274–278 2009.